Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation

Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation Publisher Pubmed

Summary: Scientists report blocking podoplanin reduces glioblastoma cell growth and spread, suggesting a new cancer therapy. #CancerResearch #BrainCancer

Gharahkhani R¹ ; Pourhadi M¹ ; Mirdamadi NS¹ ; Dana N¹ ; Rafiee L¹ ; Nedaeinia R² ; Javanmard SH¹

Source: Archives of Medical Research Published:2022

Abstract

Background: The transmembrane receptor podoplanin (PDPN) is a platelet aggregation-inducing factor, which is widely expressed in various malignant tumors such as squamous cell carcinomas, mesotheliomas, glioblastomas. Podoplanin regulates a pathway leading to cell invasion and migration. Glioblastoma multiforme (GBM) is the most aggressive and invasive tumor of the central nervous system. A high level of PDPN expression has been reported to be associated with reduced survival, cancer aggression and migration. Aim of study to determine the effect of anti-podoplanin antibody on cell-platelet aggregation, cell invasion and viability in Uppsala 87 malignant glioma (U87MG) cell lines. Methods: The expression of podoplanin on U87MG cells was measured using flowcytometry. The dimethyl-thiazol diphenyl-tetrazolium bromide (MTT) assay was used to measure U87MG cell proliferation after treatment by anti-podoplanin monoclonal antibody (NZ-1.3). The invasion of cancer cells was assessed by using a novel microfluidic based assay. Results: The results show reduction of cell viability and cell migration after treatment with anti-podoplanin antibody. After co- treatment of U87MG cells and platelets with and without anti-podoplanin antibody, the cell-platelet aggregation was significantly reduced in anti-podoplanin treated cell. Conclusions: Podoplanin is involved in aggregation of gliobastoma cells, and their viability and invasion and its neutralizing antibody can inhibit this process. So, blocking of podoplanin may be representing a promising therapeutic approach to glioblastoma multiform cancer therapy. © 2022

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Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation

Style	Citing Format
MLA	Gharahkhani R, et al.. "Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation." Archives of Medical Research, vol. 53, no. 5, 2022, pp. 461-468.
APA	Gharahkhani R, Pourhadi M, Mirdamadi NS, Dana N, Rafiee L, Nedaeinia R, Javanmard SH (2022). Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation. Archives of Medical Research, 53(5), 461-468.
Chicago	Gharahkhani R, Pourhadi M, Mirdamadi NS, Dana N, Rafiee L, Nedaeinia R, Javanmard SH. "Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation." Archives of Medical Research 53, no. 5 (2022): 461-468.
Harvard	Gharahkhani R et al. (2022) 'Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation', Archives of Medical Research, 53(5), pp. 461-468.
Vancouver	Gharahkhani R, Pourhadi M, Mirdamadi NS, Dana N, Rafiee L, Nedaeinia R, et al.. Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation. Archives of Medical Research. 2022;53(5):461-468.
BibTex	@article{ author = {Gharahkhani R and Pourhadi M and Mirdamadi NS and Dana N and Rafiee L and Nedaeinia R and Javanmard SH}, title = {Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation}, journal = {Archives of Medical Research}, volume = {53}, number = {5}, pages = {461-468}, year = {2022} }
RIS	TY - JOUR AU - Gharahkhani R AU - Pourhadi M AU - Mirdamadi NS AU - Dana N AU - Rafiee L AU - Nedaeinia R AU - Javanmard SH TI - Effect of Anti-Podoplanin on Malignant Glioma Cell Viability, Invasion and Tumor Cell-Induced Platelet Aggregation JO - Archives of Medical Research VL - 53 IS - 5 SP - 461 EP - 468 PY - 2022 ER -

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