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Preparation of a Biomimetic Bi-Layer Chitosan Wound Dressing Composed of A-Prf/Sponge Layer and L-Arginine/Nanofiber Publisher Pubmed



Mirhaj M1 ; Tavakoli M1 ; Varshosaz J2 ; Labbaf S1 ; Salehi S3 ; Talebi A4 ; Kazemi N3 ; Haghighi V5 ; Alizadeh M6
Authors

Source: Carbohydrate Polymers Published:2022


Abstract

To better mimic the structure of skin tissue, the use of a multi-layered wound dressing has been proposed. In the present study, a sponge-nanofibrous bi-layer dressing is designed. For this purpose, a chitosan/polyethylene glycol (CsPEG) sponge with advanced platelet-rich fibrin (A-PRF) was prepared as the upper layer of wound dressing, and a Cs/L–arginine electrospun nanofiber layer as the bottom layer. After physical, chemical and mechanical evaluations, the release of platelet-derived growth factor-AB (PDGF-AB), vascular endothelial growth factor (VEGF) and L-arginine were investigated. The antibacterial activity, cell viability and attachment of Bi-layer1.5 dressing (CsPEG/1.5A-PRF sponge coated with Cs/0.5 L-arginine nanofibers) were significantly higher than other dressings. Also, Bi-layer1.5 dressing increased the angiogenic potential and accelerated the wound healing, compared to other samples. Given the promising obtained results, the use of Bi-layer1.5 wound dressing with the ability to release growth factors and L-arginine is highly recommended to treat full-thickness wounds. © 2022
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