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Hypoxia-Preconditioned Wj-Msc Spheroid-Derived Exosomes Delivering Mir-210 for Renal Cell Restoration in Hypoxia-Reoxygenation Injury Publisher Pubmed

Summary: A study found enhanced exosomes from stem cells aid kidney recovery from injury. #KidneyHealth #StemCellTherapy

Toghiani R1 ; Azimian Zavareh V2 ; Najafi H1 ; Mirian M3 ; Azarpira N4 ; Abolmaali SS1 ; Varshosaz J5 ; Tamaddon AM1, 6
Authors

Source: Stem Cell Research and Therapy Published:2024


Abstract

Background: Recent advancements in mesenchymal stem cell (MSC) technology have paved the way for innovative treatment options for various diseases. These stem cells play a crucial role in tissue regeneration and repair, releasing local anti-inflammatory and healing signals. However, challenges such as homing issues and tumorigenicity have led to exploring MSC-exosomes as a promising alternative. MSC-exosomes have shown therapeutic potential in conditions like renal ischemia-reperfusion injury, but low production yields hinder their clinical use. Methods: To address this limitation, we examined hypoxic preconditioning of Wharton jelly-derived MSCs (WJ-MSCs) 3D-cultured in spheroids on isolated exosome yields and miR-21 expression. We then evaluated their capacity to load miR-210 into HEK-293 cells and mitigate ROS production, consequently enhancing their survival and migration under hypoxia-reoxygenation conditions. Results: MiR-210 overexpression was significantly induced by optimized culture and preconditioning conditions, which also improved the production yield of exosomes from grown MSCs. The exosomes enriched with miR-210 demonstrated a protective effect by improving survival, reducing apoptosis and ROS accumulation in damaged renal cells, and ultimately promoting cell migration. Conclusion: The present study underscores the possibility of employing advanced techniques to maximize the therapeutic attributes of exosomes produced from WJ-MSC spheroid for improved recovery outcomes in ischemia-reperfusion injuries. © The Author(s) 2024.
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