Immunological Diagnosis of Human Hydatid Cyst Using Western Immunoblotting Technique Publisher



Hadipour M¹ ; Nazari M² ; Sanei B² ; Ghayour Z¹ ; Sharafi SM³ ; Yazdani H¹ ; Yousofi Darani H¹
Source: Journal of Research in Medical Sciences Published:2016

Abstract

Background: Echinococcosis is a parasitic disease with worldwide distribution which is caused by the tapeworms Echinococcus granulosus. Diagnosis of the disease relies on imaging techniques, but the techniques are not able to differentiate the cyst from benign or malignant tumors; hence, appropriate serologic methods are required for the differential diagnosis of the infection. Materials and Methods: In this investigation, different sheep hydatid cyst antigens probed with thirty sera of patients with hydatid cyst and also thirty human normal sera using Western immunoblotting technique. Considering results of surgery as gold standard, sensitivity and specificity of Western blotting was estimated. Results: Sera of 29, 26, and 16 patients with hydatid cyst reacted with specific bands of hydatid cyst fluid (HCF), protoscolex crude antigen, and cyst wall crude antigen, respectively. However, none of the normal human sera reacted with those specific bands. Conclusion: A 20 kDa band of sheep HCF is an appropriate antigen for serodiagnosis of hydatid cyst infection. © 2016 Journal of Research in Medical Sciences.