3D Printed Polycaprolactone/Gelatin/Ordered Mesoporous Calcium Magnesium Silicate Nanocomposite Scaffold for Bone Tissue Regeneration Publisher Pubmed

Summary: 3D-printed PCL/gelatin/om-CMS scaffolds with 10% om-CMS show improved degradation, bioactivity, and cell viability for bone tissue regeneration. #TissueEngineering #Nanocomposites

Mirzavandi Z¹ ; Poursamar SA¹ ; Amiri F¹ ; Bigham A^{2, 3} ; Rafienia M^{1, 4}
Source: Journal of Materials Science: Materials in Medicine Published:2024

Abstract

Tissue engineering scaffolds are three-dimensional structures that provide an appropriate environment for cellular attachment, proliferation, and differentiation. Depending on their specific purpose, these scaffolds must possess distinct features, including appropriate mechanical properties, porosity, desired degradation rate, and cell compatibility. This investigation aimed to fabricate a new nanocomposite scaffold using a 3D printing technique composed of poly(ε-caprolactone) (PCL)/Gelatin (GEL)/ordered mesoporous calcium-magnesium silicate (om-CMS) particles. Different weight ratios of om-CMS were added and optimized, and a series of scaffolds were constructed for comparison purposes, including PCL 50%/Gel 50%, PCL 50%/Gel 45%/om-CMS%5, and PCL 50%/Gel 40%/om-CMS%10. The optimized weight ratio of om-CMS was 10% without leaving behind negative effects on the filaments’ structure. The scaffolds’ physical and chemical properties were assessed using various techniques, and their degradation rate, bioactivity potential, cell viability, attachment, and ALP activity were evaluated in vitro. The results demonstrated that the PCL 50%/Gel 40%/om-CMS10% scaffold had promising potential for further studies in bone tissue regeneration. Graphical Abstract: (Figure presented.). © The Author(s) 2024.