Bacterial Leakage of Bioceramic Versus Resin Sealers in Different Obturation Techniques: An In-Vitro Study Publisher

Summary: Which root canal sealer blocks bacteria better? An extracted-tooth experiment found no significant leakage difference between Neo-sealer and AH-Plus across techniques. #Dentistry #Endodontics

Akhavan A ; Farhad A ; Kashani M S A ; Tarrahi M J ; Heidari F ; Rengo C ; Milleri M ; Soltani P ; Traino F
Source: Giornale Italiano di Endodonzia Published:2026

Abstract

Aim: To compare bacterial leakage of Neo-sealer (a bioceramic sealer) and AH-Plus (an epoxy resin-based sealer) using different obturation techniques. Methodology: Ninety mandibular molars without caries, cracks, fractures, resorption, open apices, or prior endodontic treatment were selected. Mesial roots were standardized in length; distal roots were discarded. Samples were randomly divided into six groups: (1) Neo-sealer + single cone; (2) Neo-sealer + lateral compaction; (3) Neo-sealer + warm vertical compaction; (4) AH-Plus + single cone; (5) AH-Plus + lateral compaction; and (6) AH-Plus + warm vertical compaction. Canals were prepared to size #30 with 4% taper and irrigated using 5% NaOCl and saline. Each group was obturated according to its protocol. Bacterial leakage was evaluated by a blinded operator, and the time to leakage was recorded. Statistical analysis was performed using two-way ANOVA and Tukey’s test (α=0.05) Results: Mean leakage times (in days) were: 9.64, 17.53, 13.21, 12.47, 7.73, and 8.53 for groups 1–6, respectively. No significant differences were found among sealers, techniques, or their combinations (P>0.05). Conclusions: Neo-sealer and AH-Plus showed similar effectiveness in preventing coronal bacterial leakage across all obturation techniques. The single cone method was comparable to lateral and warm vertical compaction in sealing ability. © 2026 Ariesdue Srl. All rights reserved.