Cloning of Iranian Leishmania Major Mannose-1-Phosphate Guanyltransferase Gene



Hejazi SH¹ ; Ziajahromi N² ; Bandehpour M³ ; Eslami G¹ ; Salehi R⁴ ; Khamesipour A⁵ ; Kazemi B³
Source: African Journal of Biotechnology Published:2009

Abstract

Leishmania is an obligatory intracellular protozoan parasite which infects human beings when sand fly vector takes a blood meal. Most efforts are towards designing an effective vaccine to prevent Leishmanasis. In this way, development of candidate antigen for vaccine has special importance. The gene encoding mannose-1-phosphate guanyltransferase has 1140 bp and is on chromosome 23. In eukaryotes, mannose is a monosaccharide used for the glycosylation of lipids and proteins. In the absent of the enzyme, they are unable to synthesize mannose-containing glycoconjugates such as protein and lipid. In this study, we cloned mannose-1-phosphate guanyltransferase gene of Iranian L. major in pET32a expression vector. © 2009 Academic Journals.