Emerging Role of Mir-372 and Mir-101A in Head and Neck Squamous Cell Carcinoma Publisher Pubmed



Moradi N^{1, 2} ; Fadaei R³ ; Haqgou M⁴ ; Barez SR⁵ ; Kargasheh FB⁶ ; Shanaki M⁷ ; Dilmaghani NA^{4, 8}
Source: Clinical Laboratory Published:2020

Abstract

Background: microRNAs (miRNAs) have an important role in cancer development and progression. It has been shown that miR-372 and miR-101 are involved in cancer progression. In the present study we evaluated expres-sions of these miRNAs and their serum levels in patients with head and neck squamous cell carcinoma (HNSCC) and controls. Methods: We conducted this case-control study on 60 patients with HNSCC and 30 controls. Patients were diagnosed by histological assessments of their tissues. Expressions of EGFR, PTEN, PI3K/CA, miR-372, and miR-101a were evaluated in the tissues, along with serum levels of the miRNAs. Results: Tissue expression of PTEN decreased in HNSCC, and expressions of EGFR and PI3K increased in HNSCC tissues compared to the controls. Tissue expressions of miR-372 increased and miR-101a decreased in HNSCC tissues compared to the controls. We observed significantly lower serum levels of miR-101a in patients; however, these findings for miR-372 were not significant. A strong correlation existed between serum levels and tissue expression of miR-101a. Notably, miR-101a serum levels showed good sensitivity and specificity for diagnosis of HNSCC. Conclusions: The results showed that HNSCC patients had higher tissue expression of miR-372 and lower expression of miR-101a. Also, serum levels of miR-101a were lower in HNSCC patients. We observed that miR-101a had good sensitivity and specificity for diagnosis of HNSCC. The present study suggested that miR-101a could be a potential biomarker for HNSCC. © 2020 Verlag Klinisches Labor GmbH. All rights reserved.