Association Analysis of T-Cell Immunoglobulin and Mucin 1 Gene 5383-5397 Insertion/Deletion Polymorphism With Asthma and Total Serum Immunoglobulin E Levels in Patients With Asthma and Controls



Shirzad H^{1, 2} ; Meshkat R^{1, 2} ; Gangalikhanihakemi M³ ; Salehi R⁴ ; Ghasemi R^{5, 7} ; Hashemzadeh M⁶ ; Mosayebian A^{5, 7} ; Parchamibarjui S⁸
Source: Journal of Isfahan Medical School Published:2014

Abstract

Background: A member of a T-cell immunoglobulin (Ig) domain and mucin domain (TIM) gene family, TIM-1, in 5q31-33 region is shown to be correlated with development of T helper-2 (TH2) cells and allergic diseases. Polymorphism insertion/deletion (ins/del) of 5383-5397 may affect the length of TIM-1 mucin domain. The aim of this study was to investigate the association of polymorphism insertion/deletion (ins/del) 5383-5397 and susceptibility to asthma in Chaharmahal va Bakhtiari Province, Iran. Methods: Polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) were used for investigating the presence of (ins/del) 5383-5397 polymorphism in 300 patients with asthma and healthy controls. Total IgE was measured via the enzyme-linked immunosorbent assay (ELISA) technique in their serum samples as well. Findings: Significant associations were found between the insertion/insertion genotype in 5383-5397 insertion/deletion polymorphism with both asthma and levels of total serum IgE in patients with asthma (P < 0.05). Furthermore, we found that the frequency of the deletion allele is significantly lower in patients with asthma. Conclusion: Based on these findings, we identified a significant association between 5383-5397 ins/del polymorphism with asthma and with total serum IgE levels in Chaharmahal va Bakhtiari Province population. © 2014, Isfahan University of Medical Sciences(IUMS). All rights reserved.