Anti-Seizure Effects of Walnut Peptides in Mouse Models of Induced Seizure: The Involvement of Gaba and Nitric Oxide Pathways Publisher Pubmed



Jahanbani R^{1, 2} ; Bahramnejad E^{2, 3} ; Rahimi N^{2, 3} ; Shafaroodi H^{2, 3} ; Sheibani N⁴ ; Moosavimovahedi AA^{1, 5} ; Dehpour AR^{2, 3} ; Vahdati K⁶
Source: Epilepsy Research Published:2021

Abstract

Epilepsy is one of the foremost medical disorders. Oxidative stress is a well-known mechanism in epileptogenesis, and many studies suggest that oxidative stress affects the onset and evolution of epilepsy. Here we evaluated the walnut peptide extracts’ anti-seizure property in three different mouse seizure models including pentylenetetrazole-induced clonic seizure, chemical kindling, and maximal electroshock. Walnut peptides (20 mg/Kg) were administered by intraperitoneal (IP) injection of mice 60 min before seizure induction in the three models. To delineate the mechanisms of walnut peptides anti-seizure activity, we evaluated the impact of diazepam, flumazenil, and a NOS inhibitor on this activity. Intraperitoneal administration of walnut peptides significantly increased the seizure threshold. Our results also demonstrated that walnut peptides exert their anti-seizure properties through the modulation of benzodiazepine receptors. Thus, walnut peptides may be considered as a new anti-convulsion agent, which can reduce seizure occurrence and slow down seizure progression. © 2021 Elsevier B.V.