Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights

Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights Publisher Pubmed

Chamani R¹ ; Asghari SM¹ ; Alizadeh AM² ; Eskandari S¹ ; Mansouri K³ ; Khodarahmi R³ ; Taghdir M⁴ ; Heidari Z¹ ; Gorji A⁵ ; Aliakbar A⁶ ; Ranjbar B⁴ ; Khajeh K⁷

Source: Vascular Pharmacology Published:2015

Abstract

Although considerable effort has been devoted to understanding the molecular mechanism of endostatin's anti-cancer activity, the role of its Zn bound N-terminal loop has not been completely clarified. To investigate whether Zn binding or the N-terminal loop is involved in the anti-cancer properties of endostatin, we compared the structure and biological activity of a native Zn binding endostatin peptide (ES-Zn) with three variants: a Zn free variant (ES), a variant containing both a Zn binding site and a disulfide bond (ES-SSZn), and a variant including a disulfide loop but incapable of Zn binding (ES-SS). Spectroscopic studies indicated that ES-Zn and ES-SS consist of random coil and β structures, whereas ES-SSZn and ES fold into random coils. Theoretical analysis proposed that ES-Zn and ES-SS have a similar binding site to αVβ3 integrin. The anti-proliferative activity of endostatin was retained by all peptides except ES, and the in vitro anti-angiogenic property was preserved in ES-Zn and ES-SS. Remarkably, breast tumor growth and CD31 activity were inhibited more effectively by ES-SS than by ES-Zn. Therefore, a correlation exists between the N-terminal loop and anti-cancer properties of endostatin fragment and a disulfide loop may be more promising than a Zn binding loop for inhibiting tumor growth. © 2015 Elsevier Inc..

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Alimohammad Alizadeh (10)

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Style	Citing Format
MLA	Chamani R, et al.. "Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights." Vascular Pharmacology, vol. 72, no. , 2015, pp. 73-82.
APA	Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, Taghdir M, Heidari Z, Gorji A, Aliakbar A, Ranjbar B, Khajeh K (2015). Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights. Vascular Pharmacology, 72(), 73-82.
Chicago	Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, Taghdir M, et al.. "Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights." Vascular Pharmacology 72, no. (2015): 73-82.
Harvard	Chamani R et al. (2015) 'Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights', Vascular Pharmacology, 72(), pp. 73-82.
Vancouver	Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, et al.. Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights. Vascular Pharmacology. 2015;72():73-82.
BibTex	@article{ author = {Chamani R and Asghari SM and Alizadeh AM and Eskandari S and Mansouri K and Khodarahmi R and Taghdir M and Heidari Z and Gorji A and Aliakbar A and Ranjbar B and Khajeh K}, title = {Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights}, journal = {Vascular Pharmacology}, volume = {72}, number = {}, pages = {73-82}, year = {2015} }
RIS	TY - JOUR AU - Chamani R AU - Asghari SM AU - Alizadeh AM AU - Eskandari S AU - Mansouri K AU - Khodarahmi R AU - Taghdir M AU - Heidari Z AU - Gorji A AU - Aliakbar A AU - Ranjbar B AU - Khajeh K TI - Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights JO - Vascular Pharmacology VL - 72 IS - SP - 73 EP - 82 PY - 2015 ER -

Style

Citing Format

MLA

Chamani R, et al.. "Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights." Vascular Pharmacology, vol. 72, no. , 2015, pp. 73-82.

APA

Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, Taghdir M, Heidari Z, Gorji A, Aliakbar A, Ranjbar B, Khajeh K (2015). Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights. Vascular Pharmacology, 72(), 73-82.

Chicago

Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, Taghdir M, et al.. "Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights." Vascular Pharmacology 72, no. (2015): 73-82.

Harvard

Chamani R et al. (2015) 'Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights', Vascular Pharmacology, 72(), pp. 73-82.

Vancouver

Chamani R, Asghari SM, Alizadeh AM, Eskandari S, Mansouri K, Khodarahmi R, et al.. Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights. Vascular Pharmacology. 2015;72():73-82.

BibTex

@article{ author = {Chamani R and Asghari SM and Alizadeh AM and Eskandari S and Mansouri K and Khodarahmi R and Taghdir M and Heidari Z and Gorji A and Aliakbar A and Ranjbar B and Khajeh K}, title = {Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights}, journal = {Vascular Pharmacology}, volume = {72}, number = {}, pages = {73-82}, year = {2015} }

RIS

TY - JOUR
AU - Chamani R
AU - Asghari SM
AU - Alizadeh AM
AU - Eskandari S
AU - Mansouri K
AU - Khodarahmi R
AU - Taghdir M
AU - Heidari Z
AU - Gorji A
AU - Aliakbar A
AU - Ranjbar B
AU - Khajeh K
TI - Engineering of a Disulfide Loop Instead of a Zn Binding Loop Restores the Anti-Proliferative, Anti-Angiogenic and Anti-Tumor Activities of the N-Terminal Fragment of Endostatin: Mechanistic and Therapeutic Insights
JO - Vascular Pharmacology
VL - 72
IS -
SP - 73
EP - 82
PY - 2015
ER -

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