Photo-Activated Disinfection Based on Indocyanine Green Against Cell Viability and Biofilm Formation of Porphyromonas Gingivalis Publisher Pubmed



Pourhajibagher M¹ ; Chiniforush N² ; Ghorbanzadeh R³ ; Bahador A^{1, 2, 4}
Source: Photodiagnosis and Photodynamic Therapy Published:2017

Abstract

Background Photo-activated disinfection (PAD) is a novel treatment approach, in which bacteria in the root canal system may be exposed to sub-lethal doses of PAD. Such exposure can affect bacterial survival and virulence features, such as biofilm formation ability. The aim of this study was to evaluate the effects of sub-lethal doses of PAD (sPAD) using indocyanine green (ICG) on load and biofilm formation ability of Porphyromonas gingivalis as an anaerobic bacterium associated with endodontic infection. Materials and methods The anti-bacterial and anti-biofilm potential of sPAD against P. gingivalis at sub-lethal doses of ICG as a photosensitizer and using 810 nm wavelength of diode laser light via colony forming unit and crystal violet assays, respectively, was determined. Results High concentrations of ICG and light irradiation time significantly reduced bacteria. High doses of sPAD markedly reduced the number of bacteria and the formation of biofilm, up to 30.4% and 25.1%, respectively. Conclusion High doses of sPAD affected cell viability and the biofilm formation ability of P. gingivalis; lower doses did not. Thus, selection of appropriate PAD dosage should be considered for the successful treatment of endodontic in vivo. © 2016 Elsevier B.V.