Cag Pathogenicity Island-Dependent Upregulation of Matrix Metalloproteinase-7 in Infected Patients With Helicobacter Pylori Publisher Pubmed



Sadeghiani M¹ ; Bagheri N² ; Shahi H¹ ; Reiisi S³ ; Rahimian G⁴ ; Rashidi R⁴ ; Mahsa M⁴ ; Shafigh M⁴ ; Salimi E⁵ ; Rafieiankopaei M⁶ ; Hashemzadehchaleshtori M¹ ; Shirzad H¹
Source: Journal of Immunoassay and Immunochemistry Published:2017

Abstract

Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a large family of zincendopeptidases which play important roles in degradation of extracellular matrix (ECM) and various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of virulence factors, such as vacA (vacuolating cytotoxin A) and cagA (cytotoxin-associated gene), in H. pylori-infected patients upon the MMP-7 mRNA mucosal levels. We also determined the correlation between mucosal MMP-7 mRNA levels and the types of disease. Total RNA was extracted from gastric biopsies of 50 H. pylori-infected patients and 50 uninfected individuals. Mucosal MMP-7 mRNA expression level in H. pylori-infected and non-infected gastric biopsies was determined by real-time polymerase chain reaction (PCR). The presences of cagA and vacA virulence factors was evaluated using PCR. MMP-7 expression was significantly higher in biopsies of patients infected with H.pylori compared to uninfected individuals. In addition, mucosal MMP-7 mRNA expression in H. pylori-infected patients significantly associated with the cagA status and the types of disease. Our results suggest that MMP-7 might be involved in the pathogenesis of H. pylori. Peptic ulcer was associated with cag pathogenicity island-dependent MMP-7 upregulation. © 2017 Taylor & Francis.