Thiolated Chitosan-Lauric Acid As a New Chitosan Derivative: Synthesis, Characterization and Cytotoxicity Publisher Pubmed



Kazemi MS^{1, 2} ; Mohammadi Z³ ; Amini M⁴ ; Yousefi M⁵ ; Tarighi P⁶ ; Eftekhari S⁶ ; Rafiee Tehrani M¹
Source: International Journal of Biological Macromolecules Published:2019

Abstract

Chitosan as a biopolymer is an attractive vehicle for biomedical applications due to its unique characteristics. In order to improve chitosan's physicochemical features, chemical modification has been carried out to make it more suitable for such approaches. The aim of this study was to prepare and evaluate thiolated chitosan-lauric acid as a new chitosan derivative for biomedical use. Lauric acid was introduced to chitosan via stable amide bond between carboxylic acid group of fatty acid and the amine in the chitosan and thiolation was carried out using thioglycolic acid. Resulted polymers were characterized by FTIR, 1H NMR and TGA. Moreover, cell viability assessment of new derivative was performed using MTT method. FTIR and 1H NMR results showed that both substitution reactions were successfully completed. Furthermore, new synthesized polymer had no significant cytotoxicity against normal gingiva human cells (HGF1-PI 1).These findings confirm that this new derivative can be introduced as a suitable polymer for biomedical purposes such as mucosal drug delivery. © 2019 Elsevier B.V.