Design, Synthesis and Evaluation of a Prostate-Specific Antigen Rapid Test Strip Publisher



Shahri M ; Ferdosishahandashti E ; Kardar GA
Source: Journal of Babol University of Medical Sciences Published:2025

Abstract

Background and Objective: Early detection of all cancers, including prostate cancer, is considerably helpful in their treatment. In addition to clinical examination of the prostate gland, prostate-specific antigen (PSA) is also used to diagnose prostate cancer. Methods such as ELISA, PCR, radioimmunoassay, etc. have been used to detect PSA, which, in addition to being expensive, require an experienced technician, complex equipment, and a lot of time. The aim of this study is to design and produce a rapid test for PSA measurement in Iran, contribute to its production, reduce imports and prevent capital outflow. Methods: In this experimental study, colloidal gold nanoparticles were covalently conjugated with monoclonal antibodies, and different components of the diagnostic test strip were examined in terms of type and pore size, and different parts of the test strip were assembled and the antibodies were loaded onto the substrate. Serum samples from healthy subjects and patients with different serum dilutions ranging from 1 to 9 ng/mL for prostate-specific antigen were obtained from a diagnostic laboratory which had previously been measured with VIDAS. Then, the sera were measured with the designed test strip. Finally, the test strip was examined for accuracy and precision. Findings: In measuring different serum dilutions of prostate-specific antigen from 1 to 9 ng/mL, the test strip designed in this study showed similar results to the commercial kit, demonstrating the accuracy and sensitivity of the test strip designed in this study. Conclusion: A rapid prostate cancer detection test strip based on PSA measurement was designed and prototyped. © 2024 Babol University of Medical Sciences. Published by Babol University of Medical Sciences.