Investigation of the Role of Il18, Il-1Β and Nlrp3 Inflammasome in Reducing Expression of Flg-2 Protein in Psoriasis Vulgaris Skin Lesions Publisher Pubmed



Akhlaghi M¹ ; Karrabi M² ; Atabti H³ ; Raoofi A^{4, 5} ; Mousavi Khaneghah A⁶
Source: Biotechnic and Histochemistry Published:2022

Abstract

We investigated the effects of the NACHT leucine-rich repeat- and PYD-containing proteins (NLRP3) inflammasome, interleukin −18 (IL-18) and interleukin-1 beta (IL-1β) cytokines on the expression of filaggrin-2 (FLG-2) protein in psoriasis patients. Peripheral blood mononuclear cells (PBMC), including T cells, were isolated from psoriasis patients and healthy donors. Ribonucleic acid (RNA) extraction and reverse transcription-polymerase chain reaction (RT-PCR) were performed for all specimens. Immunohistochemical analysis for FLG-2 in normal and psoriatic epidermal tissue also was performed. Western blot was used to separate and identify FLG-2 protein, and immunohistochemical staining was performed to assess FLG-2 expression for psoriasis skin lesions and normal skin. RT-PCR analysis indicated that NLRP3 inflammasome, IL-18 cytokine and IL‐1β cytokine expression were increased in psoriatic epidermis compared to normal skin. We found that the expression of FLG-2 was decreased in psoriatic epidermis compared to normal skin. Higher levels of NLRP3 help decrease the FLG-2 level. © 2021 The Biological Stain Commission.