Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1

Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1 Publisher

Fathabad ME¹ ; Karimipoor M² ; Alizadeh S¹ ; Abdoli A³ ; Atashi A⁴ ; Sayadi M¹

Source: BioImpacts Published:2017

Abstract

Introduction: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder caused by the Philadelphia chromosome translocation, at (9; 22), which results in BCR-ABL fusion tyrosine kinase oncoprotein. This fusion induces down-regulation of miR-155. Upregulation of miR-155 can influence cell fate via the effect on p27kip1 and apoptosis. The aim of this study was to induce apoptosis in K562 CML cell line by overexpression of miR- 155. Methods: The K562 cell line was transfected with pLenti-III-pre mir155-GFP constructs through electroporation. Then, overexpression of miR-155 as well as the expression level of p27kip1 and c-Myc was analyzed by quantitative PCR (qPCR). The level of p27 (Kip1) protein expression was measured by Western blot and the Annexin V method was carried out to investigate apoptosis. Results: Flow cytometric analysis results of K562 cells transfected with pLenti-III-pre mir155- GFP construct showed a significant increase in cell apoptosis. Gene expression and protein level of p27kip1 were upregulated. However, there was no change in c-Myc expression profile. Conclusion: miR-155 could be a promising approach to aid in the treatment of CML. However, further studies are required in this respect. © 2017 The Author(s).

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Style	Citing Format
MLA	Fathabad ME, et al.. "Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1." BioImpacts, vol. 7, no. 2, 2017, pp. 109-114.
APA	Fathabad ME, Karimipoor M, Alizadeh S, Abdoli A, Atashi A, Sayadi M (2017). Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1. BioImpacts, 7(2), 109-114.
Chicago	Fathabad ME, Karimipoor M, Alizadeh S, Abdoli A, Atashi A, Sayadi M. "Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1." BioImpacts 7, no. 2 (2017): 109-114.
Harvard	Fathabad ME et al. (2017) 'Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1', BioImpacts, 7(2), pp. 109-114.
Vancouver	Fathabad ME, Karimipoor M, Alizadeh S, Abdoli A, Atashi A, Sayadi M. Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1. BioImpacts. 2017;7(2):109-114.
BibTex	@article{ author = {Fathabad ME and Karimipoor M and Alizadeh S and Abdoli A and Atashi A and Sayadi M}, title = {Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1}, journal = {BioImpacts}, volume = {7}, number = {2}, pages = {109-114}, year = {2017} }
RIS	TY - JOUR AU - Fathabad ME AU - Karimipoor M AU - Alizadeh S AU - Abdoli A AU - Atashi A AU - Sayadi M TI - Mir-155 Effectively Induces Apoptosis in K562 Philadelphia Positive Cell Line Through Upregulation of P27kip1 JO - BioImpacts VL - 7 IS - 2 SP - 109 EP - 114 PY - 2017 ER -

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