Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Folic Acid Ameliorates Palmitate-Induced Inflammation Through Decreasing Homocysteine and Inhibiting Nf-Κb Pathway in Hepg2 Cells Publisher Pubmed



Bagherieh M1 ; Kheirollahi A2 ; Zamanigarmsiri F1 ; Emamgholipour S1 ; Meshkani R1
Authors

Source: Archives of Physiology and Biochemistry Published:2023


Abstract

Objective: Prevention of inflammation is one of the possible remedy procedure for steatohepatitis during NAFLD. In this study, we researched the folic acid (FA) potency to attenuate the inflammation of palmitate-treated HepG2 cells and the related signalling pathways. Methods: The molecular mechanisms related to FA anti-inflammatory effect in palmitate and Hcy-treated HepG2 cell line were assessed. Results: The results indicated that while palmitate enhances the expression and secretion of TNF-α, IL-6, and IL-1β, and also intracellular ROS level, FA at concentrations of 25, 50, and 75 µg/mL significantly reversed these effects in HepG2 cells. In addition, FA could ameliorate inflammation and decrease ROS production induced by Hcy. Furthermore, FA pre-treatment suppress palmitate -induced (NF-κB) p65 level in palmitate or Hcy stimulated cells. Conclusions: Overall, these results suggest that FA reduces inflammation in HepG2 cells through decreasing ROS and Hcy concentration level resulting in inhibiting the NF-κB pathway. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Effects of Folic Acid Supplementation on Inflammatory Markers: A Grade-Assessed Systematic Review and Dose–Response Meta-Analysis of Randomized Controlled Trials, Nutrients (2021)
2. High Glucose Induces Inflammatory Responses in Hepg2 Cells Via the Oxidative Stress-Mediated Activation of Nf-Κb, and Mapk Pathways in Hepg2 Cells, Archives of Physiology and Biochemistry (2018)
3. Unlocking the Therapeutic Potential of Autophagy Modulation by Natural Products in Tackling Non-Alcoholic Fatty Liver Disease, Phytotherapy Research (2025)
Experts (# of related papers)
View all Related Experts
Reza Meshkani (7)
Ghodratollah Panahi (3)
Other Related Docs
4. The Effects of Folic Acid Supplementation on Pro-Inflammatory Mediators: A Systematic Review and Dose–Response Meta‐Analysis of Randomized Controlled Trials, Clinical Therapeutics (2021)
5. Interplay Between Oxidative Stress and Autophagy Function and Its Role in Inflammatory Cytokine Expression Induced by Palmitate in Skeletal Muscle Cells, Cytokine (2020)
6. The Effects of Guluronic Acid (G2013), a New Emerging Treatment, on Inflammatory Factors in Nonalcoholic Steatohepatitis Patients Under in Vitro Conditions, Immunopharmacology and Immunotoxicology (2021)
7. Toll-Like Receptors and Metabolic (Dysfunction)-Associated Fatty Liver Disease, Pharmacological Research (2022)
8. Therapeutic Potential of Fisetin in Hepatic Steatosis: Insights Into Autophagy Pathway Regulation and Endoplasmic Reticulum Stress Alleviation in High-Fat Diet-Fed Mice, PLoS ONE (2025)
9. Synbiotic Supplementation in Lean Patients With Non-Alcoholic Fatty Liver Disease: A Pilot, Randomised, Double-Blind, Placebo-Controlled, Clinical Trial, British Journal of Nutrition (2017)
10. Curcumin Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Regulating Jnk/Nf-Kb Pathway and Ros Production, Inflammopharmacology (2018)
11. Effect of Curcumin on the Expression of Nod2 Receptor and Pro-Inflammatory Cytokines in Fibroblast-Like Synoviocytes (Flss) of Rheumatoid Arthritis (Ra) Patients, Advances in Rheumatology (2023)
12. Thymol Reduces Acetic Acid-Induced Inflammatory Response Through Inhibition of Nf-Kb Signaling Pathway in Rat Colon Tissue, Inflammopharmacology (2019)
13. Combinational Administration of Mesenchymal Stem Cell-Derived Exosomes and Metformin Reduces Inflammatory Responses in an in Vitro Model of Insulin Resistance in Hepg2 Cells, Heliyon (2023)
14. Combination Therapy of Metformin and P-Coumaric Acid Mitigates Metabolic Dysfunction Associated With Obesity and Nonalcoholic Fatty Liver Disease in High-Fat Diet Obese C57bl/6 Mice, Journal of Nutritional Biochemistry (2023)
15. Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and Jnk/Nf-Κb Pathway in a Sirt1-Independent Mechanism, Journal of Cellular Biochemistry (2017)
16. The Evaluation of Safety Property and Apoptotic Efficacy of Α-L-Guluronic Acid (G2013), As a Novel Nsaid, Under in Vitro Examination on L929 and Hepatocellular Carcinoma Cell Lines, Recent Advances in Inflammation and Allergy Drug Discovery (2021)
17. Signaling Pathways of Melatonin in Prevention of Liver Disorders Via Suppressing of Oxidative Stress in Cellular Level, Biomedical and Pharmacology Journal (2016)
18. Nod-Like Receptors in the Pathogenesis of Metabolic (Dysfunction)-Associated Fatty Liver Disease: Therapeutic Agents Targeting Nod-Like Receptors, Diabetes and Metabolic Syndrome: Clinical Research and Reviews (2023)
19. Anti-Inflammatory Function of Apolipoprotein B-Depleted Plasma Is Impaired in Nonalcoholic Fatty Liver Disease, PLoS ONE (2022)
20. Recent Advances in Dietary Supplementation, in Treating Non-Alcoholic Fatty Liver Disease, World Journal of Hepatology (2015)