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Bioinformatics and Experimental Insights Into Linc01605 and the Mir-101–3P/Birc5 Axis in Oral Squamous Cell Carcinoma Pathogenesis and Clinical Outcomes Publisher Pubmed



Abbasalipourkabir R ; Aslani S ; Morovat S ; Ziamajidi N ; Akrami E ; Pourvahdani S ; Bahmani M ; Razavi AE ; Kalantarycharvadeh A
Authors

Source: Cancer Treatment and Research Communications Published:2026


Abstract

Background: LncRNAs modulate several druggable and non-druggable genes through miRNAs sequestering in oral squamous cell carcinoma (OSCC). This study evaluates LINC01605 expression and its potential interaction with the miR-101–3p/BIRC5 axis in OSCC development. Methods: The OSCC sequencing data were downloaded from The Cancer Genome Atlas database. Different online databases, along with correlation analysis, were employed to predict lncRNAs downstream miRNAs and mRNAs. Further protein-protein interaction network and functional analysis were conducted to discover the role of target genes. The expression analysis through RT-qPCR, western blotting, and immunofluorescence, as well as correlation analysis of the axis, was studied in 30 paired OSCC and margin tissues. ROC, Kaplan-Meier, and Chi-square analyses were used to estimate the diagnostic, prognostic, and histopathological utility of this axis, respectively. Results: After novelty checking and downstream finding, LINC01605/miR-101–3p/BIRC5 was selected. RT-qPCR showed upregulation of LINC01605, alongside the downregulation of miR-101–3p. The mRNA and protein levels of BIRC5 exhibited elevated expression in OSCC tissues. Significant positive correlation between LINC01605 and birc5, along with negative correlations between LINC01605-miR-101–3p, and miR-101–3p-birc5 was found. The combined ROC curve demonstrated a high discriminating potential of this axis in OSCC tissue diagnosis. Moreover, patients with high LINC01605 and low miR-101–3p levels had a significantly poorer survival, and this was associated with perineural invasion and invasion depth. Conclusion: This study reveals a new axis related to OSCC and may provide beneficial diagnostic, prognostic, and potential therapeutic targets in this cancer. © 2026 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/
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