Anti-Toxoplasma Activity of 2-(Naphthalene-2-Γlthiol)-1H Indole



Asgari Q¹ ; Keshavarz H² ; Rezaeian M² ; Sadeghpour H^{3, 4} ; Miri R^{3, 4} ; Motazedian MH¹
Source: Iranian Journal of Parasitology Published:2015

Abstract

Background: This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1Hindole. Methods: Tachyzoites of RH strain were incubated in various concentrations of 2-(naphthalene-2-ylthio)-1H-indole (25-800µM) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS). To evaluate the infectivity, the tachyzoites exposed to the different concentrations of the compound were inoculated to 10 BALB/c mice groups. For Control, parasites exposed to DMSO (0.2% v/v) were also intraperitoneally inoculated into two groups of mice. The immunity of the exposed tachyzoites was evaluated by inoculation of the naive parasite to the survived mice. Results: The LD50 of 2-(naphthalene-2-ylthio)-1H-indole was 57 µmol. The longevity of mice was dose dependent. Five mice out of group 400µmol and 3 out of group 800µmol showed immunization to the parasite. Conclusion: Our findings demonstrated the toxoplasmocidal activity of the compound. The presence of a well-organized transporter mechanism for indole compounds within the parasite in conjunction with several effective mechanisms of these compounds on Toxoplasma viability would open a window for production of new drugs and vaccines. © 2015, Tehran University of Medical Sciences (TUMS). All rights reserved.