Protective Effects of Hydroalcoholic Extracts From an Ancient Apple Variety 'Mela Rosa Dei Monti Sibillini' Against Renal Ischemia/Reperfusion Injury in Rats Publisher Pubmed



Yousefimanesh H^{1, 2, 3} ; Hemmati S¹ ; Shirooie S⁴ ; Nabavi SM⁵ ; Talebzadeh Bonakdar A¹ ; Fayaznia R² ; Hossein Asgardoon M⁶ ; Zare Dehnavi A³ ; Ghafouri M³ ; Nkuimi Wandjou JG⁷ ; Caprioli G⁷ ; Sut S⁸ ; Maggi F⁷ ; Dallacqua S⁹
Source: Food and Function Published:2019

Abstract

The purpose of this work was to investigate the effect of hydroalcoholic extracts from the peel (APE) and pulp (APP) of a traditional apple variety of central Italy, the 'Mela Rosa dei Monti Sibillini', on the damage caused by renal ischemia/reperfusion injury (IRI) in rats. Thirty mg per kg b.w. of the extracts were administered intraperitoneally to male adult Wistar rats 3 days before the induction of IRI by pedicle clamping. A significant decrease in the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNFα), interleukin 1 beta (IL-1β) and nuclear factor-κB (NF-κB) was observed in the groups pre-treated with APE when compared with IRI rats. The chemical composition of APE was determined by HPLC-DAD-MSn highlighting a significant amount of proanthocyanidins (52.9 mg g-1), flavonols (42.27 mg g-1) and dihydrochalcones (11.75 mg g-1). These findings indicated that this ancient apple variety is a promising source of nutraceuticals and functional foods helpful to manage complications of renal disorders. © 2019 The Royal Society of Chemistry.