Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein

Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein Publisher Pubmed

Bianchini F¹ ; Crivelli V¹ ; Abernathy ME² ; Guerra C¹ ; Palus M^{3, 4} ; Muri J¹ ; Marcotte H⁵ ; Piralla A⁶ ; Pedotti M¹ ; De Gasparo R¹ ; Simonelli L¹ ; Matkovic M¹ ; Toscano C¹ ; Biggiogero M⁷ Show All Authors

Bianchini F¹
Crivelli V¹
Abernathy ME²
Guerra C¹
Palus M^{3, 4}
Muri J¹
Marcotte H⁵
Piralla A⁶
Pedotti M¹
De Gasparo R¹
Simonelli L¹
Matkovic M¹
Toscano C¹
Biggiogero M⁷
Calvaruso V⁷
Svoboda P^{3, 4, 8, 9}
Rincon TC¹
Fava T¹
Podesvova L¹
Shanbhag AA¹
Celoria A¹
Sgrignani J¹
Stefanik M^{4, 10}
Honig V^{3, 4}
Pranclova V^{3, 11}
Michalcikova T¹²
Prochazka J¹²
Guerrini G¹³
Mehn D¹³
Ciabattini A¹⁴
Abolhassani H^{5, 15}
Jarrossay D¹
Uguccioni M¹
Medaglini D¹⁴
Panhammarstrom Q⁵
Calzolai L¹³
Fernandez D¹⁶
Baldanti F^{6, 17}
Franzettipellanda A⁷
Garzoni C¹⁸
Sedlacek R¹²
Ruzek D^{3, 4, 8}
Varani L¹
Cavalli A^{1, 19}
Barnes CO^{2, 20}
Robbiani DF¹

Source: Science Immunology Published:2023

Abstract

Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2′ site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are cross-reactive with Orthocoronavirinae, including SARS-CoV-2 variants. Copyright © 2023 The Authors, some rights reserved.

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Style	Citing Format
MLA	Bianchini F, et al.. "Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein." Science Immunology, vol. 8, no. 81, 2023, pp. -.
APA	Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, Marcotte H, Piralla A, Pedotti M, De Gasparo R, Simonelli L, Matkovic M, Toscano C, Biggiogero M, Calvaruso V, Svoboda P, Rincon TC, Fava T, Podesvova L, ... Robbiani DF (2023). Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein. Science Immunology, 8(81), -.
Chicago	Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, Marcotte H, et al.. "Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein." Science Immunology 8, no. 81 (2023): -.
Harvard	Bianchini F et al. (2023) 'Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein', Science Immunology, 8(81), pp. -.
Vancouver	Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, et al.. Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein. Science Immunology. 2023;8(81):-.
BibTex	@article{ author = {Bianchini F and Crivelli V and Abernathy ME and Guerra C and Palus M and Muri J and Marcotte H and Piralla A and Pedotti M and De Gasparo R and Simonelli L and Matkovic M and Toscano C and Biggiogero M and Calvaruso V and Svoboda P and Rincon TC and Fava T and Podesvova L and Shanbhag AA and Celoria A and Sgrignani J and Stefanik M and Honig V and Pranclova V and Michalcikova T and Prochazka J and Guerrini G and Mehn D and Ciabattini A and Abolhassani H and Jarrossay D and Uguccioni M and Medaglini D and Panhammarstrom Q and Calzolai L and Fernandez D and Baldanti F and Franzettipellanda A and Garzoni C and Sedlacek R and Ruzek D and Varani L and Cavalli A and Barnes CO and Robbiani DF}, title = {Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein}, journal = {Science Immunology}, volume = {8}, number = {81}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Bianchini F AU - Crivelli V AU - Abernathy ME AU - Guerra C AU - Palus M AU - Muri J AU - Marcotte H AU - Piralla A AU - Pedotti M AU - De Gasparo R AU - Simonelli L AU - Matkovic M AU - Toscano C AU - Biggiogero M AU - Calvaruso V AU - Svoboda P AU - Rincon TC AU - Fava T AU - Podesvova L AU - Shanbhag AA AU - Celoria A AU - Sgrignani J AU - Stefanik M AU - Honig V AU - Pranclova V AU - Michalcikova T AU - Prochazka J AU - Guerrini G AU - Mehn D AU - Ciabattini A AU - Abolhassani H AU - Jarrossay D AU - Uguccioni M AU - Medaglini D AU - Panhammarstrom Q AU - Calzolai L AU - Fernandez D AU - Baldanti F AU - Franzettipellanda A AU - Garzoni C AU - Sedlacek R AU - Ruzek D AU - Varani L AU - Cavalli A AU - Barnes CO AU - Robbiani DF TI - Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein JO - Science Immunology VL - 8 IS - 81 SP - EP - PY - 2023 ER -

Style

Citing Format

MLA

Bianchini F, et al.. "Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein." Science Immunology, vol. 8, no. 81, 2023, pp. -.

APA

Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, Marcotte H, Piralla A, Pedotti M, De Gasparo R, Simonelli L, Matkovic M, Toscano C, Biggiogero M, Calvaruso V, Svoboda P, Rincon TC, Fava T, Podesvova L, ... Robbiani DF (2023). Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein. Science Immunology, 8(81), -.

Chicago

Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, Marcotte H, et al.. "Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein." Science Immunology 8, no. 81 (2023): -.

Harvard

Bianchini F et al. (2023) 'Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein', Science Immunology, 8(81), pp. -.

Vancouver

Bianchini F, Crivelli V, Abernathy ME, Guerra C, Palus M, Muri J, et al.. Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein. Science Immunology. 2023;8(81):-.

BibTex

@article{ author = {Bianchini F and Crivelli V and Abernathy ME and Guerra C and Palus M and Muri J and Marcotte H and Piralla A and Pedotti M and De Gasparo R and Simonelli L and Matkovic M and Toscano C and Biggiogero M and Calvaruso V and Svoboda P and Rincon TC and Fava T and Podesvova L and Shanbhag AA and Celoria A and Sgrignani J and Stefanik M and Honig V and Pranclova V and Michalcikova T and Prochazka J and Guerrini G and Mehn D and Ciabattini A and Abolhassani H and Jarrossay D and Uguccioni M and Medaglini D and Panhammarstrom Q and Calzolai L and Fernandez D and Baldanti F and Franzettipellanda A and Garzoni C and Sedlacek R and Ruzek D and Varani L and Cavalli A and Barnes CO and Robbiani DF}, title = {Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein}, journal = {Science Immunology}, volume = {8}, number = {81}, pages = {-}, year = {2023} }

RIS

TY - JOUR
AU - Bianchini F
AU - Crivelli V
AU - Abernathy ME
AU - Guerra C
AU - Palus M
AU - Muri J
AU - Marcotte H
AU - Piralla A
AU - Pedotti M
AU - De Gasparo R
AU - Simonelli L
AU - Matkovic M
AU - Toscano C
AU - Biggiogero M
AU - Calvaruso V
AU - Svoboda P
AU - Rincon TC
AU - Fava T
AU - Podesvova L
AU - Shanbhag AA
AU - Celoria A
AU - Sgrignani J
AU - Stefanik M
AU - Honig V
AU - Pranclova V
AU - Michalcikova T
AU - Prochazka J
AU - Guerrini G
AU - Mehn D
AU - Ciabattini A
AU - Abolhassani H
AU - Jarrossay D
AU - Uguccioni M
AU - Medaglini D
AU - Panhammarstrom Q
AU - Calzolai L
AU - Fernandez D
AU - Baldanti F
AU - Franzettipellanda A
AU - Garzoni C
AU - Sedlacek R
AU - Ruzek D
AU - Varani L
AU - Cavalli A
AU - Barnes CO
AU - Robbiani DF
TI - Human Neutralizing Antibodies to Cold Linear Epitopes and Subdomain 1 of the Sars-Cov-2 Spike Glycoprotein
JO - Science Immunology
VL - 8
IS - 81
SP -
EP -
PY - 2023
ER -

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