In Vitro and in Vivo Study of Carboxymethyl Chitosan/Polyvinyl Alcohol for Wound Dressing Application Publisher



Akbari A¹ ; Rabbani S² ; Irani S¹ ; Zandi M³ ; Sharifi F⁴ ; Ameli F⁵ ; Mohamadali M¹
Source: Journal of Applied Polymer Science Published:2022

Abstract

Antibacterial, good biosafety, and wound healing enhancers, are the most favorable factors for wound dressing. Designing nanomaterial of the scaffold for supporting skin regeneration is a complicated process. Carboxymethyl chitosan (CMC) structure similar to the glycosaminoglycan and antibacterial activity combined with polyvinyl alcohol (PVA) (PVA/CMC) can lead to satisfactory properties for wound management. In this study, a collection of electrospun nanofibrous scaffolds are fabricated by blending the constant PVA concentration (9%) with a limited area of CMC concentration (2%, 3%, 4%, and 5%). PVA/CMC scaffolds characteristic was investigated through a scanning electron microscope, Fourier-transform infrared spectroscopy, and contact angle tests. The fiber diameter values were measured for PVA (144.60 nm), chitosan (131.57 nm), and average different concentration of CMC in PVA/CMC (104.7 ± 2.45 nm). In vitro potential of the scaffolds were evaluated by using human dermal fibroblast cells and human placental-derived mesenchymal stem cells. According to in vitro results, PVA/CMC4% scaffold was selected as the optimized wound dressing for skin tissue engineering. The in vivo wound healing data in rat models illustrated more healing capacity of PVA/CMC4% dressing in which accelerating wound closure and freshly healed tissue with high similarity to the normal skin was reorganized. © 2021 Wiley Periodicals LLC.