Evaluation of Mir-210 Expression in Common Variable Immunodeficiency: Patients With Unsolved Genetic Defect Publisher Pubmed



Babaha F^{1, 2} ; Yazdani R² ; Shahkarami S^{2, 3, 4} ; Esfahani ZH^{1, 2} ; Abolhahassani H^{2, 5} ; Sadr M⁶ ; Hosseini AZ¹ ; Aghamohammadi A²
Source: Allergologia et Immunopathologia Published:2021

Abstract

Background: Common variable immunodeficiency (CVID) is one of the most prevalent forms of primary immunodeficiency diseases (PID). CVID is characterized by failure in the final differentiation of B lymphocytes and impaired antibody production but the pathogenesis is not known in the majority of patients. We postulated that the expression pattern of miRNAs in unsolved CVID patients might be the underlying epigenetic cause of the disease. Therefore, we aimed to assess the expression of hsa-miR-210-5p and FOXP3 transcription factor in CVID cases in comparison with healthy individuals. Methods: Eleven CVID cases with no genetic defects (all PID known genes excluded) and 10 sex and age-matched healthy individuals were enrolled in the study. T lymphocytes were purified from PBMC, and expression levels of miR-210-5p and FOXP3 mRNA were evaluated by real-time PCR. Results: We demonstrated that miR-210 expression in patients was significantly higher than the control group (P = 0.03). FOXP3 expression was slightly lower in patients compared with healthy controls (P = 0.86). There was a negative correlation between miR and gene expression (r: -0.11, P = 0.73). Among various clinical complications, autoimmunity showed a considerable © 2021. Codon Publications. Published by Codon Publications. All Rights Reserved.