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Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells Publisher



Simonian M1 ; Ghaffari MH1 ; Salimi A2 ; Mirzadegan E3 ; Sadeghi N2 ; Ebrahimnezhad N2 ; Fazli G2 ; Fatemi R2 ; Bayat AA2 ; Milani S4 ; Negahdari B1 ; Rabbani H2
Authors

Source: Avicenna Journal of Medical Biotechnology Published:2022


Abstract

Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines. Conclusion: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies. © 2022, Avicenna Journal of Medical Biotechnology. All rights reserved.
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