An Evaluation of Photobiomodulation Effects on Human Gingival Fibroblast Cells Under Hyperglycemic Condition: An in Vitro Study Publisher Pubmed



Iranpour B¹ ; Mohammadi K¹ ; Hodjat M² ; Hakimiha N³ ; Sayar F¹ ; Kharazi Fard MJ² ; Sadatmansouri S¹ ; Hanna R^{4, 5, 6}
Source: Lasers in Medical Science Published:2024

Abstract

An in vitro study was designed to evaluate the effects of photobiomodulation (PBM) with 915-nm diode laser on human gingival fibroblast (HGF) cells under hyperglycemic condition. The HGF cells were cultured in Dulbecco’s modified eagle medium (DMEM) medium containing 30 mM glucose concentration for 48 h to mimic the hyperglycemic condition. Subsequently, the cells received three sessions of PBM (915 nm, continuous emission mode, 200 mW, energy density values of 3.2, 6, and 9.2 J/cm2). Twenty-four hours post-irradiation, cell proliferation, expression of interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) were assessed with MTT and real-time polymerase chain reaction (PCR) tests, respectively. Also, reactive oxygen species (ROS) production was measured using CM-H2DCFDA fluorimetry. No changes were detected in the cell proliferation rate between the high glucose control group and laser-treated cells, while VEGF and IL-6 gene expression levels increased significantly after PBM in the high glucose-treated cells group. ROS level was significantly decreased in the irradiated cells in high-glucose medium compared with the high glucose control group. Our study revealed the inductive role of 915-nm–mediated PBM on VEGF and the inflammatory response while concurrently reducing reactive oxygen species production in HGF cells in hyperglycemic conditions. © 2023, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.