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The Effect of Testosterone and Antioxidants Nanoliposomes on Gene Expressions and Sperm Parameters in Asthenospermic Individuals Publisher Pubmed



Mohammadzadeh M1 ; Hamishehkar H2 ; Vatanparast M3 ; Akhavan Sales Z4 ; Nabi A5 ; Mazaheri F6 ; Mohseni F7, 8 ; Talebi AR6
Authors

Source: Drug Development and Industrial Pharmacy Published:2021


Abstract

Background: Antioxidants that used for the infertility treatment cannot have their complete effectiveness, because of their instability in the culture medium. Significance: One of the most advances, in the drug delivery systems, is nanoliposomes-loaded, as biodegradable and bioavailable carriers. Hormonal and antioxidant agents encapsulating inside the nanoliposomes were used, to increase the effectiveness of antioxidants in the sperm culture medium. Materials: Semen sample from 15 asthenospermia were divided into 10 equal parts. After preparation, the sperms were incubated with free form of drugs and nanocarriers contained resveratrol, catalase, resveratrol-catalase and testosterone for 45 min. All sperm parameters, sperm DNA and gene expressions were evaluated before and after freezing. Results: Before freezing, all nanocarriers and free testosterone showed higher sperm motility compared to free drugs (p=.000). Free Testosterone and free resveratrol-catalase had higher DNA damage compared to nanocarriers (p=.000). Before freezing, the blank nanoliposome and testosterone nanoliposomes had the lowest HSP70 gene expression respectively (p = 0.005) (p = 0.001). After freezing, a significant reduction in sperm motility was observed in the free resveratrol-catalase group (p=.003). Also, a significant increase in sperm viability was observed in the free testosterone and nanoliposomes of blank and testosterone (p > 0.05). The least DNA fragmentation was related to catalase nanoliposomes (p=.000). All nanoliposomes, especially catalase, had the highest percentage of class I morphology compared to the control group (p=.000). Conclusions: Nanoliposomes could improve the sperm parameters and DNA integrity before and after freezing, by increasing the effectiveness of antioxidants. So, it can be recommended in the ART lab. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
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