The Protective Effect of Zataria Multiflora on the Embryotoxicity Induced by Bisphenol a in the Brain of Chicken Embryos Publisher



Najafi PZ¹ ; Ashrafizadeh M² ; Farkhondeh T^{1, 3} ; Peivastehroudsari L^{4, 5} ; Samarghandian S⁶
Source: Biointerface Research in Applied Chemistry Published:2019

Abstract

Bisphenol A (BPA), is one of the main industrial chemicals synthesized for various purposes. In the present study, the brain tissue of chicken embryos was used to evaluate the embryotoxic effects of BPA and also the preventive effects of Zataria multiflora. To that end, fertile eggs were categorized into four groups (n=10). The eggs air sacs of the experimental groups were injected BPA (200 ppm) after four days. The Zataria multiflora (100 and 200 μg/egg) was administered into chick embryos 6 hours prior to administration of BPA. The control group simply was treated with olive oil. The eggs were incubated at 37°C and a humidity of 63%. After 20 days, the embryos were beheaded and the brains were gathered for biochemical measurements. The findings indicated that Zataria multiflora (200 μg/egg) significantly reversed the increased levels of MDA (p<0.05) and protein (p<0.001) in the brain of BPA-exposed group and also the decreased levels of total antioxidant and GSH as well as the CAT and SOD activities in the brain of BPA-exposed group. Zataria multiflora reversed the toxic effects of BPA on the embryonic development stages of the brain via modulating oxidative stress. © 2019 by the authors.