Apoferritin-Templated Biosynthesis of Manganese Nanoparticles and Investigation of Direct Electron Transfer of Mnnps–Hsafr at Modified Glassy Carbon Electrode Publisher Pubmed



Rafipour R¹ ; Kashanian S^{2, 6} ; Hashemi S³ ; Omidfar K^{4, 7} ; Ezzati Nazhad Dolatabadi J⁵
Source: Biotechnology and Applied Biochemistry Published:2017

Abstract

Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide–N-hydroxysuccinimide and glutaraldehyde coupling. The MnNPs–HsAFr immobilized on CNT-CS/GC electrode was characterized by cyclic voltammetry. This charge transfer coefficient (α) and the exchange current (i0) of MnNPs–HsAFr immobilized on modified electrode in 0.1 M phosphate solution (pH 7.5) were found to be 0.57 and 0.48 μA, respectively. © 2015 International Union of Biochemistry and Molecular Biology, Inc.