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Rapid Method for the Determination of Cyclophosphamide and Ifosphamide in Urine at Trace Levels by Gas Chromatography-Mass Spectrometry



Rastkari N1 ; Ahmadkhaniha R2 ; Alehashem M3 ; Baniasadi S3
Authors

Source: International Journal of Pharmaceutical and Clinical Research Published:2016

Abstract

Introduction: The oxazaphosphorines cyclophosphamide (CY) and Ifosfamide (IF) are amongst the most widely used drugs in cancer chemotherapy. In both routine cancer therapy and experimental cancer research, many people might be exposed to CY and IF during pharmaceutical handling of the agents, so developing of a reliable analysis method for biomonitoring is necessary. Materials and Methods: A rapid and simple method for the simultaneous extraction of CP and IP from human urine has been developed using liquid-liquid extraction. The detection limits of cyclophosphamide and ifosphamide in urine samples were 0.5 and 1 ng ml-1, respectively, with a signal-to-noise ratio of 3:1. The regression correlation coefficients (r2) were over 0.99 in all experiments. Conclusion: This method is sensitive enough to determination of low levels of CP and IF in a range of urine concentrations relevant to performing low exposure assessment. © 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved.
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