Altered Expression of Malat 1 Lncrna in Chronic Lymphocytic Leukemia Patients, Correlation With Cytogenetic Findings Publisher



Ahmadi A¹ ; Kaviani S¹ ; Yaghmaie M^{2, 3, 4} ; Pashaiefar H^{2, 3, 4} ; Ahmadvand M^{2, 3, 4} ; Jalili M^{2, 3, 4} ; Alimoghaddam K^{2, 3, 4} ; Eslamijouybari M⁵ ; Ghavamzadeh A^{2, 3, 4}
Source: Blood Research Published:2018

Abstract

Background: Recent studies have devoted much attention to non-protein-coding transcripts in relation to a wide range of malignancies. MALAT1, a long non-coding RNA, has been reported to be associated with cancer progression and prognosis. Thus, we here determined MALAT1 gene expression in chronic lymphocytic leukemia (CLL), a genetically heterogeneous disease, and explored its possible relationships with cytogenetic abnormalities. Methods: MALAT1 expression level was evaluated using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) on blood mononuclear cells from 30 non-treated CLL patients and 30 matched healthy controls. Cytogenetic abnormalities were determined in patients by fluorescence in situ hybridization (FISH). Results: MALAT1 expression level was up-regulated in the CLL group compared to healthy controls (P=0.008). Del13q14, followed by Del11q22, were the most prevalent cytogenetic abnormalities. We found no association between the FISH results and MALAT1 expression in patients. Conclusion: Altered expression of MALAT1 is associated with CLL development. Further investigations are required to assess the relationship between this long non-coding RNA and CLL patient survival and prognosis. © 2018 Korean Society of Hematology.