The Pre-Mir-499 Variant Rs3746444 May Contribute to Coronary Artery Disease Susceptibility: A Case-Control and Meta-Analysis Study Publisher Pubmed



Labbaf A¹ ; Ghaedi I¹ ; Alipoor B² ; Omrani MD³ ; Kazcrouni F¹ ; Shanaki M¹ ; Ghaffarzadeh M¹ ; Pashaiefar H⁴ ; Rahimipour A¹
Source: Clinical Laboratory Published:2017

Abstract

Background: Recent reports have suggested that single-nucleotide polymorphisms (SNPs) in microRNA genes may contribute to individual susceptibility to coronary artery disease (CAD). The purpose of this study was to evaluate the association between rs3746444 in pre-miR-499 with CAD. Methods: We performed a case-control study including 288 CAD patients and 150 control subjects. DNA was extracted from blood samples and genotyped through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Meta-analysis was performed under fixed effect and random effect models whenever appropriate. Results: We found that the GG genotype is significantly more frequent in CAD patients than controls (adjusted p = 0.010; OR = 1.99, 95%; CI: 1.18 - 3.38). Additionally, through a meta-analysis, we showed that miR-499- rs3746444 has a significant association with cardiovascular disease. Conclusions: Our results suggest that miR-499-rs3746444-GG is associated with CAD susceptibility and development.