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The Safety Property of P-D-Mannuronic Acid (M2000) As a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells Publisher Pubmed



Fard NA1 ; Tabrizian N1 ; Mirzaei R2 ; Motamed N3 ; Zavareh FT4 ; Mirshafiey A4
Authors

Source: Current Drug Discovery Technologies Published:2016


Abstract

The study's background and aim: In this investigation, the safety property of M2000 (P-D-mannuronic acid) on differentiation, maturation and function of dendritic cells, was determined. P-D-mannuronic acid, as a novel immunosuppressive and antiinflammatory agent, has been tested in various experimental models. In addition, DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, although, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. Materials and Methods: The effect of M2000 on differentiation, maturation and function of dendritic cells was examined. To investigate how M2000 affects human dendritic cells (DC) in a defined inflammatory environment, human peripheral blood mononuclear cells (PBMC) were isolated from healthy blood and monocytes were purified using anti-CD14 microbeads. Monocytes were incubated with M2000 in two different doses (6 and 12 ^g/well) along with adding the granulocyte-macrophage colony-stimulating factor (GM- CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC maturation. The differentiation, maturation and function of dendritic cells were examined with flow cytometry and ELISA method. Result: The results demonstrate that M2000 has no significant side on differentiation, maturation and function of dendritic cells in immature DC and mature DC process in vitro. Conclusion: Our findings show that P-D-mannuronic acid (m2000) as a safe agent had no adverse effect on differentiation, maturation and function of dendritic cells which might be recommended as a novel immunosuppressive agent with no or fewer side effects in increasing the risk of infectious diseases and cancers. © 2016 Bentham Science Publishers.
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