Inactivated Herpes Simplex Virus-1 Vaccine Formulated in Aqueous and Alcoholic Extracts of Propolis Boosts Cellular and Igg Responses Publisher



Mojarab S^{1, 2} ; Karimi P³ ; Shahbazzadeh D⁴ ; Moghbeli M² ; Bagheri KP⁴ ; Parikhani AB⁴ ; Dehghan R⁴ ; Zafari E¹ ; Moravej A⁵ ; Pouriayevali MH^{6, 7} ; Mirtalebi SF⁸ ; Pakjoo M¹ ; Yazdani S^{9, 10} ; Abdollahpouralitappeh M¹¹ Show All Authors
Source: Iranian Journal of Basic Medical Sciences Published:2024

Abstract

Objective(s): In this study, the adjuvant activity of aqueous and alcoholic extracts of propolis was examined on the inactivated herpes simplex virus-1 (HSV-1). Materials and Methods: BALB/C mice were administered with inactivated (HSV-1; the KOS strain) plus alcoholic and aqueous extracts, followed by assessment of the cellular and humoral immune responses. Results: Alcoholic and aqueous extracts, as an adjuvant, revealed a significant increase in lymphocyte proliferation and cytotoxic T lymphocyte (CTL) responses versus the HSV-1 group. In addition, HSV-1 plus alcoholic extract showed a remarkable increase in IFN-γ cytokine and IFN-γ/IL-4 ratio. On the other hand, both alcoholic and aqueous extracts in the HSV-1 vaccine suppressed the IL-4 cytokine response as compared with the HSV-1 vaccine. In addition, HSV-1 plus alcoholic extract showed a significant increment in IgG1, IgG2a, and IgG2b isotypes as compared with the HSV-1 vaccine. Conclusion: Propolis extracts seem to modulate the immune response against inactivated HSV-1 model and can be used as a suitable vaccine adjuvant or a component of a complex adjuvant against infectious diseases. © 2024 Mashhad University of Medical Sciences. All rights reserved.