Identification of Germline Monoallelic Mutations in Ikzf2 in Patients With Immune Dysregulation Publisher Pubmed



Shahin T^{1, 2, 3, 4} ; Mayr D^{1, 2, 3} ; Shoeb MR² ; Kuehn HS⁵ ; Hoeger B^{1, 2, 3} ; Giuliani S^{1, 2} ; Gawriyski LM⁶ ; Petronczki OY^{1, 2, 3} ; Hadjadj J⁷ ; Bal SK^{1, 2, 3} ; Zoghi S^{1, 2, 3, 8, 9} ; Haimel M^{1, 2, 3} ; Heredia RJ^{1, 2, 3, 4} ; Boutboul D¹⁰ Show All Authors
Source: Blood Advances Published:2022

Abstract

Helios, encoded by IKZF2, is a member of the Ikaros family of transcription factors with pivotal roles in T-follicular helper, NK- and T-regulatory cell physiology. Somatic IKZF2 mutations are frequently found in lymphoid malignancies. Although germline mutations in IKZF1 and IKZF3 encoding Ikaros and Aiolos have recently been identified in patients with phenotypically similar immunodeficiency syndromes, the effect of germline mutations in IKZF2 on human hematopoiesis and immunity remains enigmatic. We identified germline IKZF2 mutations (one nonsense (p.R291X)- and 4 distinct missense variants) in six patients with systemic lupus erythematosus, immune thrombocytopenia or EBV-associated hemophagocytic lymphohistiocytosis. Patients exhibited hypogammaglobulinemia, decreased number of T-follicular helper and NK cells. Single-cell RNA sequencing of PBMCs from the patient carrying the R291X variant revealed upregulation of proinflammatory genes associated with T-cell receptor activation and T-cell exhaustion. Functional assays revealed the inability of HeliosR291X to homodimerize and bind target DNA as dimers. Moreover, proteomic analysis by proximity-dependent Biotin Identification revealed aberrant interaction of 3/5 Helios mutants with core components of the NuRD complex conveying HELIOS-mediated epigenetic and transcriptional dysregulation. © 2022 by The American Society of Hematology.