Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Distinguishing Echinococcus Granulosus Sensu Stricto Genotypes G1 and G3 With Confidence: A Practical Guide Publisher Pubmed



Kinkar L1 ; Laurimae T1 ; Acostajamett G2 ; Andresiuk V3 ; Balkaya I4 ; Casulli A5 ; Gasser RB6 ; Gonzalez LM7 ; Haag KL8 ; Zait H9 ; Irshadullah M10 ; Jabbar A6 ; Jenkins DJ11 ; Manfredi MT12 Show All Authors
Authors
  1. Kinkar L1
  2. Laurimae T1
  3. Acostajamett G2
  4. Andresiuk V3
  5. Balkaya I4
  6. Casulli A5
  7. Gasser RB6
  8. Gonzalez LM7
  9. Haag KL8
  10. Zait H9
  11. Irshadullah M10
  12. Jabbar A6
  13. Jenkins DJ11
  14. Manfredi MT12
  15. Mirhendi H13
  16. Mrad S14
  17. Rostaminejad M15
  18. Oudnimrad M14
  19. Pierangeli NB16
  20. Poncegordo F17
  21. Rehbein S18
  22. Sharbatkhori M19
  23. Kia EB20
  24. Simsek S21
  25. Soriano SV16
  26. Sprong H22
  27. Snabel V23
  28. Umhang G24
  29. Varcasia A25
  30. Saarma U1

Source: Infection# Genetics and Evolution Published:2018


Abstract

Cystic echinococcosis (CE), a zoonotic disease caused by tapeworms of the species complex Echinococcus granulosus sensu lato, represents a substantial global health and economic burden. Within this complex, E. granulosus sensu stricto (genotypes G1 and G3) is the most frequent causative agent of human CE. Currently, there is no fully reliable method for assigning samples to genotypes G1 and G3, as the commonly used mitochondrial cox1 and nad1 genes are not sufficiently consistent for the identification and differentiation of these genotypes. Thus, a new genetic assay is required for the accurate assignment of G1 and G3. Here we use a large dataset of near-complete mtDNA sequences (n = 303) to reveal the extent of genetic variation of G1 and G3 on a broad geographical scale and to identify reliable informative positions for G1 and G3. Based on extensive sampling and sequencing data, we developed a new method, that is simple and cost-effective, to designate samples to genotypes G1 and G3. We found that the nad5 is the best gene in mtDNA to differentiate between G1 and G3, and developed new primers for the analysis. Our results also highlight problems related to the commonly used cox1 and nad1. To guarantee consistent identification of G1 and G3, we suggest using the sequencing of the nad5 gene region (680 bp). This region contains six informative positions within a relatively short fragment of the mtDNA, allowing the differentiation of G1 and G3 with confidence. Our method offers clear advantages over the previous ones, providing a significantly more consistent means to distinguish G1 and G3 than the commonly used cox1 and nad1. © 2018 Elsevier B.V.
Other Related Docs