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A Label-Free Dna Biosensor for Determination of Topotecan As an Anticancer Drug: Electrochemical, Spectroscopic and Docking Studies Publisher



Akbari Javar H1 ; Mahmoudimoghaddam H2
Authors

Source: Journal of the Electrochemical Society Published:2020


Abstract

In this study electrochemical and spectrophotometry techniques and docking tools have been utilized for studying the interaction between topotecan as an anticancer drug and ds-DNA. Moreover, a new DNA biosensor on the basis of carbon paste electrode modified with Eu3+ doped NiO (ds-DNA/Eu3+ doped NiO/CPE) provided to detect topotecan. Therefore, an experimental design based on response surface methodology (RSM), has been used to examine and optimize the impacts of diverse experimental parameters like the pH, accumulation potential, accumulation time, and concentration of the DNA for construction of biosensor. Hence, linear range of topotecan has been defined in a range between 0.09 and 110.0 μM, and limit of detection 0.04 μM has been calculated for topotecan. It has been also found that our suggested biosensor has a number of benefits such as simplicity, rapidness, reasonable reproducibility and lengthy stability. © 2020 The Electrochemical Society (ECS). Published on behalf of ECS by IOP Publishing Limited.
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