Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Clonal Relatedness and Resistance Characteristics of Oxa-24 and -58 Producing Carbapenem-Resistant Acinetobacter Baumannii Isolates in Tehran, Iran Publisher Pubmed



Salehi B1 ; Ghalavand Z2 ; Mohammadzadeh M3 ; Maleki DT2 ; Kodori M2 ; Kadkhoda H2
Authors

Source: Journal of Applied Microbiology Published:2019


Abstract

Aims: The present study was conducted to investigate the mechanism of carbapenem resistance and the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates collected from two nearby hospitals in Tehran, Iran. Methods and Results: A total of 180 CRAB isolates were studied. Antimicrobial susceptibility testing was performed using disk diffusion and Epsilometer tests. The detection of OXA-23, -24 and -58 was implemented for all isolates using polymerase chain reaction. Subsequently, isolates harbouring OXA-24 and -58 were investigated for the presence of resistance determinants of Ambler class A, metallo-β-lactamases (MBLs), and carbapenem-hydrolysing class D β-lactamases, ISAba1, and the genetic relatedness between them was analysed using pulsed-field gel electrophoresis (PFGE). All isolates were found to be resistant to imipenem with a MIC of ≥8 µg ml−1 and were susceptible to colistin with a MIC of ≤1·5 µg ml−1. Sixty percent of the isolates had OXA-23. OXA-24 and -58 were detected in 31 of 180 CRAB isolates. These chosen isolates were devoid of MBLs and blaSHV, blaC TX-M, blaVEBESBL genes. The PER determinant was detected in 38% of isolates as the most common extended spectrum β-lactamases (ESBLs). Of these isolates, 51·6% had OXA-23, and ISAba1 was found to be upstream of OXA-23 and OXA-51 in 16 and 8 isolates, respectively. The band patterns produced by PFGE showed nine clonal pulsotypes distributed between the two hospitals. Conclusion: The findings showed that the refractory CRAB isolates were transmitted intra- and inter-hospital, particularly in the ICU due to shortcomings in infection control surveillance. Significance and Impact of the Study: Carbapenem resistance is a substantial threat in the treatment of infections caused by A. baumannii due to limitations in the therapeutic options. © 2019 The Society for Applied Microbiology
Related Docs
View other Related Docs
1. Clonal Relatedness and Biofilm Formation of Oxa-23-Producing Carbapenem Resistant Acinetobacter Baumannii Isolates From Hospital Environment, Microbial Pathogenesis (2016)
2. Prevalence of Β-Lactamase-Encoding Genes and Molecular Typing of Acinetobacter Baumannii Isolates Carrying Carbapenemase Oxa-24 in Children, Annals of Clinical Microbiology and Antimicrobials (2021)
3. Plasmid Borne Carbapenem-Hydrolyzing Class D Β-Lactamases (Chdls) and Adeabc Efflux Pump Conferring Carbapenem-Tigecycline Resistance Among Acinetobacter Baumannii Isolates Harboring Tnabars, Microbial Pathogenesis (2017)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (4)
Masoumeh Douraghi (4)
Other Related Docs
4. Wide Distribution of Carbapenem Resistant Acinetobacter Baumannii in Burns Patients in Iran, Frontiers in Microbiology (2015)
5. Geographical Mapping and Temporal Trends of Acinetobacter Baumannii Carbapenem Resistance: A Comprehensive Meta-Analysis, PLoS ONE (2024)
6. First Report of Oxa-143-Lactamase Producing Acinetobacter Baumannii in Qom, Iran, Iranian Journal of Basic Medical Sciences (2017)
7. Prevalence and Mechanisms of Carbapenem Resistance in Acinetobacter Baumannii: A Comprehensive Systematic Review of Cross-Sectional Studies From Iran, Microbial Drug Resistance (2020)
8. Status of Carbapenem-Resistant Acinetobacter Baumannii Harboring Carbapenemase: First Systematic Review and Meta-Analysis From Iran, Infection# Genetics and Evolution (2019)
9. Sub-Minimum Inhibitory Concentrations (Sub-Mics) of Colistin on Acinetobacter Baumannii Biofilm Formation Potency, Adherence, and Invasion to Epithelial Host Cells: An Experimental Study in an Iranian Children’S Referral Hospital, Microbiology Spectrum (2024)
10. High Prevalence of Oxa-Type Carbapenemases Among Acinetobacter Baumannii Strains in a Teaching Hospital of Tehran, Acta Microbiologica et Immunologica Hungarica (2017)
11. Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones, Infection and Drug Resistance (2020)
12. Emergence and Characterization of Nosocomial Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter Baumannii Isolates in Tehran, Iran, Journal of Infection and Chemotherapy (2018)
13. Genotypic and Antimicrobial Susceptibility of Carbapenem-Resistant Acinetobacter Baumannii: Analysis of Is Aba Elements and Blaoxa-23-Like Genes Including a New Variant, Frontiers in Microbiology (2015)
14. Trend of Extensively Drug-Resistant Acinetobacter Baumannii and the Remaining Therapeutic Options: A Multicenter Study in Tehran, Iran Over a 3-Year Period, Letters in Applied Microbiology (2016)
15. Variable Spontaneous Mutation Rate in Clinical Strains of Multidrug-Resistant Acinetobacter Baumannii and Differentially Expressed Proteins in a Hypermutator Strain, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis (2017)
16. Evidence of Interruption of the Comm Gene in a Large Series of Clinical Isolates of Multidrug-Resistant Acinetobacter Baumannii, Journal of Molecular Microbiology and Biotechnology (2016)
17. Investigation of Epsa, Ompa, and Bap Genes Among Mdr and Xdr Acinetobacter Baumannii Isolates in Khorramabad, Iran, Recent Advances in Anti-Infective Drug Discovery (2024)
18. Antimicrobial Resistance Profiles and Genetic Elements Involved in Carbapenem Resistance in Acinetobacter Baumannii Isolates From a Referral Hospital in Southern Iran, Journal of Global Antimicrobial Resistance (2016)
19. High Prevalence of Extensively Drug-Resistant and Metallo Beta-Lactamase-Producing Clinical Acinetobacter Baumannii in Iran, Microbial Pathogenesis (2016)
20. Antimicrobial Resistance and Molecular Epidemiology of Virulence Genes Among Multi-Drug Resistant Acinetobacter Baumannii Clinical Isolates in Iran, Gene Reports (2021)