Evaluation of Regulatory T Lymphocytes and Il2ra and Foxp3 Gene Expression in Peripheral Mononuclear Cells From Patients With Amyotrophic Lateral Sclerosis Publisher Pubmed



Rashid Chehreh Bargh S¹ ; Tafakhori A^{2, 3} ; Masoumi F¹ ; Rahmani F⁴ ; Ahmadi M² ; Namdar A¹ ; Azimi M¹ ; Tavasolian P¹ ; Habibi S¹ ; Zamani B⁵ ; Maserrat M² ; Sadr M⁶ ; Noorbakhsh F¹ ; Rezaei N^{1, 6, 7, 8}
Source: Irish Journal of Medical Science Published:2018

Abstract

Background: Loss of neuroprotective role of CD4+ helper T cells, regulatory T cells, and M2 microglia constitutively results in the rapid neural death in the “rapidly progressive phase” of amyotrophic lateral sclerosis (ALS). Aim: We aimed to investigate relative count of CD4+ and regulatory T lymphocytes and expression level of IL2Ra and FOXP3 genes in peripheral blood mononuclear cells (PBMCs) from patients with ALS. Method: We performed a flow cytometric analysis on PBMC from 38 patients with ALS and 32 controls to determine the count of CD4+ and CD4+CD25+ cells. Quantitative real-time PCR analyses were implemented to determine the level of expression of FOXP3 and IL-2Rα (CD25) genes in the peripheral blood mononucleated cells. Results: We found a significant higher proportion of CD4+ T cells (p value < 0.001), along with a significantly reduced proportion of CD4+CD25+ Treg cells (p value = 0.001, p value = 0.02), in the peripheral blood of patient’s with ALS. Conclusion: The results of our study are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. This was reflected in higher peripheral percentage of CD4+ helper T cells and higher expression of FOXP3 and IL-2Rα. The observed demise in the number of active CD4+CD25+ regulatory T cells might indicate early signs of progression to later stages of ALS in our study group. Interestingly, disease duration was the sole independent significant determining factor that predicted CD4+CD25+ regulatory T cell counts in the peripheral blood of patients at various stages of ALS, according to a logistic regression model. © 2018, Royal Academy of Medicine in Ireland.