Fibrinogen and Fibronectin Binding Activity and Immunogenic Nature of Choline Binding Protein M



Afshar D¹ ; Pourmand MR^{1, 2} ; Jedditehrani M³ ; Saboor Yaraghi AA¹ ; Azarsa M¹ ; Shokri F⁴
Source: Iranian Journal of Public Health Published:2016

Abstract

Background: Choline-binding proteins (CBPs) are a group of surface-exposed proteins, which play crucial and physi-ological roles in Streptococcus pneumoniae. The novel member of CBPs, choline-binding protein M (CbpM) may have binding activity to plasma proteins. This study aimed to clone and express CbpM and demonstrate its interaction with plasma proteins and patients’ sera. Methods: The total length of cbpM gene was cloned in pET21a vector and expressed in BL21 expression host. Verifi-cation of recombinant protein was evaluated by Western blot using anti-His tag monoclonal antibody. Binding ability of the recombinant protein to plasma proteins and the interaction with patients’ sera were assessed by Western blot and ELISA methods. Results: The cbpM gene was successfully cloned into pET21a and expressed in BL21 host. Binding activity to fibron-ectin and fibrinogen and antibody reaction of CbpM to patients’ sera was demonstrated by Western blot and ELISA methods, respectively. Conclusion: CbpM is one of the pneumococcal surface-exposed proteins, which mediates pneumococcal binding to fibronectin and fibrinogen proteins. © 2016, Iranian Journal of Public Health. All rights reserved.