Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Formation of Self-Assembled Triple-Layered Rotavirus-Like Particles (Tlrlps) by Constitutive Co-Expression of Vp2, Vp6, and Vp7 in Stably Transfected High-Five Insect Cell Lines Publisher Pubmed



Shoja Z1, 2 ; Tagliamonte M3 ; Jalilvand S2 ; Mollaeikandelous Y2 ; De Stradis A4 ; Tornesello ML3 ; Buonaguro FM3 ; Buonaguro L3
Authors

Source: Journal of Medical Virology Published:2015


Abstract

In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity. © 2014 Wiley Periodicals, Inc.
Other Related Docs
4. Rotavirus Vp6 As a Potential Vaccine Candidate, Reviews in Medical Virology (2019)
14. Diversity of Vp7 Genes of G1 Rotaviruses Isolated in Iran, 2009-2013, Infection# Genetics and Evolution (2016)