Association of Angiotensin-Converting Enzyme (Ace) I/D Variation With Biochemical Parameters and Oxidative Stress Markers in Systemic Lupus Erythematosus Patients in West of Iran Publisher Pubmed



Kiani A¹ ; Eliehalikomi D^{2, 3} ; Bahrehmand F⁴ ; Mostafaei S⁵ ; Vaisiraygani A⁶ ; Baniamerian H⁶ ; Aghaz F⁶ ; Tanhapour M⁷ ; Shakiba E⁸ ; Rahimi Z⁴ ; Pourmotabbed T⁹
Source: Molecular Biology Reports Published:2023

Abstract

Purpose: We aimed to study insertion/deletion (I/D) variation (rs4646994) of ACE gene in a group of SLE patients in west of Iran and its possible relationship with oxidative stress. Method and results: Genotypes and allele frequencies related to ACE (I/D) variation were determined in 108 SLE patients and 110 gender and age-matched healthy controls using PCR. Neopterin, malondialdehyde (MDA), and serum lipid concentrations were determined by HPLC and enzyme assay respectively. The overall distribution of ACE I/D genotypes in SLE patients was different from that of the control group (P = 0.005). DD genotype compared to ID genotype increased the risk of SLE (OR = 2.57, 95% CI 1.4–4.8, P = 0.003). ID genotype compared to the II genotype decreased the risk of disease (OR = 0.45, 95% CI 0.2–0.99, p = 0.042). SLE patients with DD, ID, and II genotypes had lower paraoxonase (PON) activity and higher serum levels of MDA and neopterin versus control patients. We also detected a significant protective effect against SLE in presence of ACE I alleles and lack of angiotensin II receptor, type 1 (AGTR1) A1166C (NCBI reference SNP id: rs5186), C alleles in this study (OR = 0.31, 95% CI 0.14–0.68, P = 0.002). Conclusions: Carriers of the DD genotype of ACE gene with higher serum concentrations of neopterin and MDA, and lower PON activity had a high risk to develop SLE, while ID genotype decreased the risk of disease development by 2.22 times compared to II genotype. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.