Echinococcus Granulosus Sensu Stricto in Dogs and Jackals From Caspian Sea Region, Northern Iran



Gholami S¹ ; Jahandar H² ; Abastabar M³ ; Pagheh A⁴ ; Mobedi I⁵ ; Sharbatkhori M^{6, 7}
Source: Iranian Journal of Parasitology Published:2016

Abstract

Background: The aim of the present study was genotyping of Echinococcus gra-nulosus isolates from dogs and jackals in Mazandaran Province, northern Iran, and using partial sequence of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). Methods: E. granulosus isolates (n = 15) were collected from 42 stray dogs and 16 jackals found in south of the Caspian Sea in northern Iran. After morpho-logical study, the isolates were genetically characterized using consensus se-quences (366bp) of the cox1 gene. Phylogenetic analysis of cox1 nucleotide se-quence data was performed using a Bayesian Inference approach. Results: Four different sequences were observed among the isolates. Two genotypes [G1 (66.7%) and G3 (33.3%)] were identified among the isolates. The G1 sequences indicated three sequence profiles. One profile (Maz1) had 100% homology with reference sequence (AN: KP339045). Two other pro-files, designated Maz2 and Maz3, had 99% homology with the G1 genotype (ANs: KP339046 and KP339047). A G3 sequence designated Maz4 showed 100% homology with a G3 reference sequence (AN: KP339048). Conclusion: The occurrence of the G1 genotype of E. granulosus sensu stricto as a frequent genotype in dogs is emphasized. This study established the first mo-lecular characterization of E. granulosus in the province. © 2016, Tehran University of Medical Sciences (TUMS). All rights reserved.