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Investigating the Presence of Haemophilus Influenzae in Patients With Chronic Obstructive Pulmonary Disease (Copd) Using Molecular Method Publisher



Mohagheghmortazavi H1 ; Bahrami N2 ; Naji T3 ; Mohamadnia A4
Authors

Source: Journal of Isfahan Medical School Published:2019


Abstract

Background: Chronic obstructive pulmonary disease (COPD) is a type of obstructive pulmonary disease characterized by chronic airway blockage. The aim of this study was to investigate the presence of Haemophilus influenzae in patients with COPD using molecular method. Methods: A total of 100 samples of respiratory secretion were collected from patients with COPD. The molecular identification of Haemophilus influenza was performed using specific primers against standard kits by polymerase chain reaction (PCR) method. Findings: Haemophilus influenzae bacteria were positive in 10 out of 100 patients, and 8 of 100 were positive in PCR using specific primers. Conclusion: Nucleic acid-like PCR tests, due to their sensitivity, high accuracy, and time saving, are the appropriate method for diagnosis of Haemophilus influenzae in patients with chronic pulmonary obstruction. © 2019 Isfahan University of Medical Sciences(IUMS). All rights reserved.
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