Preparation of Etoposide Loaded Poly Butyl Cyano Acrylate Nanoparticles and It's Effect on Human Brain Carcinoma Cells Be(2)-C



Jokar F¹ ; Vakili MH¹ ; Ghadernejad H² ; Asadifar M³ ; Abdolahpour S⁴ ; Izadi M⁵ ; Moradisardareh H⁴ ; Afrisham R⁴ ; Kooti W⁶
Source: Der Pharmacia Lettre Published:2016

Abstract

The main purpose of this studywas preparation of a pharmaceutical formulation with etoposide loaded Poly Butyl Cyano Acrylate (PBCA) nanoparticles and evaluation of its effect on brain cancer. Etoposide loaded nanocarrier was synthesized using micro-emulsion polymerization method and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, BE (2)-C brain cell line was used to determine the rate of nanocarrieretoposide cytotoxicity. Etoposide loaded nanoparticles were characterized and size and zeta potential of nanoparticles containing drugs were measured as 225.3±21.7 nm and -26.3 ±1.5 mv, respectively. Drug loading and encapsulation values were estimated 8.7±0.15 and 97.3±2.1%, respectively. The release amount of drug from PBCA nanoparticles indicated the retention strength corresponding to the formulation. Cytotoxicity studies showed that the cell toxicity effect of drug loaded PBCA nanoparticles is almost two times higher than the free drugs. Finally, it was found that the obtained formulation has a proper stability and drug maintain its stability after storage in 37° for two months. According to the obtained results, this nanoparticle has a potential to be used as a drug delivery system.