Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Comparative Analysis of 198 Sars-Cov-2 Genomes From Iran and West Asia, February 2020 to December 2021 Publisher



Karamipour S1 ; Mojbafan M1, 2 ; Nezhad Fard RM3
Authors

Source: Iranian Journal of Pathology Published:2023


Abstract

Background & Objective: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulting in a worldwide pandemic. The first case of COVID-19 was reported from Wuhan in the Hubei Province of China in December 2019; however, the disease's origin is still mysterious. Whole-genome sequence analysis is essential for monitoring the spread of infectious diseases as well as studying the pathogenesis and evolution of viruses. In this study, analysis of 198 fully sequenced genomes from Iran and West Asia was carried out to study mutations, phylogeny, amino acid changes, clades, and lineages of these genomes as well as comparison of these sequences with those of reference Wuhan genome of NC_045512.2. Methods: In total, 198 completely sequenced genome data from Iran and West Asia were collected from GenBank. Mutation detection was carried out using a trial version of CLC Genomics Workbench v.21.0 (QIAGEN, Germany). Online tools such as GISAID Mutations App and Pangolin were used for further analysis of the results. Results: In this study, several unique mutation sites were identified in the Iranian genomes (n = 8); positions 1397 G>A and 29742 G>T were the most frequent changes in more than 85% of the Iranian genomes. Mutation rate, mutation per sequence, and transition versus transversion for the Iranian genomes included 4.73, 14.14, and 1.6, respectively. Generally, C>T alteration was the most common substitution in all the sequences. Conclusion: The ORF1ab, N, and S were the genes with the most changes. The current data can help researchers predict future epidemics and establish better strategies to control viral pandemics. © 2023, Iranian Society of Pathology. All rights reserved.
Other Related Docs
4. Covid-19 Infection and Stroke Risk, Reviews in the Neurosciences (2021)
8. Variable Clinical Manifestations of Covid-19: Viral and Human Genomes Talk, Iranian Journal of Allergy# Asthma and Immunology (2020)
11. Ten Challenging Questions About Sars-Cov-2 and Covid-19, Expert Review of Respiratory Medicine (2020)
12. Insights Into the Sars-Cov2 Outbreak; the Great Global Challenge: A Mini Review, Journal of Cellular and Molecular Anesthesia (2020)
13. Coronavirus: Pure Infectious Disease or Genetic Predisposition, Advances in Experimental Medicine and Biology (2021)
16. Host Factors: Implications in Immunopathogenesis of Covid-19, Pathology Research and Practice (2021)
18. Transmission Modes of Covid-19: A Systematic Review, Infectious Disorders - Drug Targets (2021)