Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients

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Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients Publisher Pubmed

Akhtari M^{1, 2} ; Vojdanian M¹ ; Javinani A¹ ; Ashrafganjouei A¹ ; Jamshidi A¹ ; Mahmoudi M^{1, 3}

Source: Cytokine Published:2020

Abstract

Background: Ankylosing spondylitis (AS) is an auto-inflammatory debilitating disorder with a complex pathogenesis. The adenosinergic pathway is an immunologic regulating pathway with a potential role in AS pathophysiology. In the present study, we have aimed to investigate the influence of A2A adenosine receptor (A2AAR) activation on tumor necrosis factor-α (TNF-α) and interleukin-23 (IL-23) expression and secretion by monocyte-generated macrophages of AS patients. Methods: Whole-blood separated monocytes were extracted from 14 AS patients and 14 healthy controls. Macrophages were differentiated by macrophage colony-stimulating factor (M-CSF), and surface markers were confirmed by flow cytometer. Cells were treated with CGS-21680 as a known agonist of A2AAR. Analysis of ADORA2A, TNFA, and IL23A gene expression was performed by SYBR green real-time PCR. The concentration of secreted cytokines was also measured by ELISA kits. Results: Based on our analysis, CGS-21680 significantly decreased TNF-α secretion by monocyte-derived macrophages of AS patients. Moreover, A2AAR agonist increased the IL23A mRNA expression level in monocyte-derived macrophages of AS patients considerably. Whereas, CGS-21680 did not have any influence on macrophages of healthy individuals. Conclusion: According to our results, it appears that A2AAR activation can increase IL-23 secretion by monocyte-derived macrophages of AS patients. Although the TNF-α reducing effect of A2AAR agonists can be a potential target in AS treatment, robust increasing of IL-23 should be considered as the undesirable effect of these agents. © 2020 Elsevier Ltd

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Style	Citing Format
MLA	Akhtari M, et al.. "Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients." Cytokine, vol. 128, no. , 2020, pp. -.
APA	Akhtari M, Vojdanian M, Javinani A, Ashrafganjouei A, Jamshidi A, Mahmoudi M (2020). Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients. Cytokine, 128(), -.
Chicago	Akhtari M, Vojdanian M, Javinani A, Ashrafganjouei A, Jamshidi A, Mahmoudi M. "Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients." Cytokine 128, no. (2020): -.
Harvard	Akhtari M et al. (2020) 'Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients', Cytokine, 128(), pp. -.
Vancouver	Akhtari M, Vojdanian M, Javinani A, Ashrafganjouei A, Jamshidi A, Mahmoudi M. Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients. Cytokine. 2020;128():-.
BibTex	@article{ author = {Akhtari M and Vojdanian M and Javinani A and Ashrafganjouei A and Jamshidi A and Mahmoudi M}, title = {Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients}, journal = {Cytokine}, volume = {128}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Akhtari M AU - Vojdanian M AU - Javinani A AU - Ashrafganjouei A AU - Jamshidi A AU - Mahmoudi M TI - Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients JO - Cytokine VL - 128 IS - SP - EP - PY - 2020 ER -