Experimental Study on Ostrich Acellular Dermal Matrix in Repair of Full-Thickness Wounds of Guinea Pig; [Kobaylarda Tum-Deri Katmanlarini Iceren Yaralarda Devekusu Aselluler Dermal Matriksin Etkisinin Arastirildigi Deneysel Bir Calisma] Publisher



Khomejani Farahani F¹ ; Fattahian H¹ ; Kajbafzade AM²
Source: Kafkas Universitesi Veteriner Fakultesi Dergisi Published:2015

Abstract

ADM (Acellular dermal matrix) can be a standard treatment for full-thickness wound defects. The aim of the present study was to achieve a decellularized matrix of the ostrich as a biomedical application and assess the evaluation of wound healing, which were implanted with ADM. Eighteen (n=18) guinea pigs were conducted and they received a full- thickness 2×2 cm flank skin wounds, nine experiment were implanted with acellular ostrich dermal matrix as experimental group and nine experiment as control group, which implanted with normal ostrich skin. At days of 3, 7 and 26 were evaluated for epithelialization and the other histological characteristics (Kruskal-wallis and Mann Whitney U tests), and the wounds were assessed for wound contracture at 26 days after surgery (ANOVA and Tukey test). Each dermal substitute decreased wound contracture and show no significant different at the endpoint of study (P˃0.05). Between groups, increasing infiltration of fibroblast and new blood vessels under collagen scaffold showed no significant different (P=0.06). Histological evaluation of wound epithelialization and neo-dermis which was produced by ADM, was thicker neo-dermis at the end of study in experimental group. The study revealed that ostrich acellular dermal matrix can be used as a temporary substitute with acceptable result in healing of full-thickness wounds. © 2015 Veteriner Fakultesi Dergisi. All rights reserved.