Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency

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Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency Publisher

Youssefian L^{1, 2} ; Saeidian AH^{2, 3} ; Saffarian Z^{4, 5} ; Ariamanesh M⁶ ; Abdollahimajd F⁷ ; Molkara S⁸ ; Shahididadras M⁷ ; Diab R⁷ ; Vahidnezhad F⁹ ; Zeinali S¹⁰ ; Beziat V^{11, 12, 13} ; Jouanguy E^{11, 12, 13} ; Casanova JL^{11, 12, 13, 14, 15} ; Uitto J¹⁶ Show All Authors

Youssefian L^{1, 2}
Saeidian AH^{2, 3}
Saffarian Z^{4, 5}
Ariamanesh M⁶
Abdollahimajd F⁷
Molkara S⁸
Shahididadras M⁷
Diab R⁷
Vahidnezhad F⁹
Zeinali S¹⁰
Beziat V^{11, 12, 13}
Jouanguy E^{11, 12, 13}
Casanova JL^{11, 12, 13, 14, 15}
Uitto J¹⁶
Vahidnezhad H^{2, 17, 18, 19}

Source: JID Innovations Published:2024

Abstract

Most viral infections can be self-limited, with no requirement for medical intervention. However, the same viruses can cause severe diseases in patients with compromised immunity due to single-gene diseases, acquired immune deficiency syndrome, or hematologic malignancies or those receiving immunosuppressive drugs. Occasionally, these immunocompromised patients harbor >1 infectious agent, requiring several concomitant diagnostic tests. We have developed, to our knowledge, a previously unreported whole-transcriptome sequencing–based pipeline that allows virome profiling, quantitation, and expression pattern analysis of 926 distinct viruses by sequencing of RNA isolated from a single lesional skin biopsy. This pipeline can also explore host genetics if there is a Mendelian predisposition to infection. We applied this pipeline to 6 Iranian patients with viral-induced skin lesions associated with immune deficiency secondary to HIV, human T-lymphotropic virus 1, chronic lymphocytic leukemia, and post transplant immunosuppression. In 5 cases, definitive human papillomavirus infections were identified, some caused by multiple viral types. In addition to human papillomavirus, coinfection with other viruses (Merkle cell polyomavirus, cytomegalovirus, and human herpesvirus 4) was detected in some lesions. In 1 case, whole-transcriptome sequencing validated the clinical diagnosis of adult T-cell leukemia/lymphoma in a patient with an initial diagnosis of mycosis fungoides/Sezary syndrome. These findings attest to the power of whole-transcriptome sequencing in profiling the cutaneous virome in the context of compromised immunity. © 2024 The Authors

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Style	Citing Format
MLA	Youssefian L, et al.. "Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency." JID Innovations, vol. 4, no. 4, 2024, pp. -.
APA	Youssefian L, Saeidian AH, Saffarian Z, Ariamanesh M, Abdollahimajd F, Molkara S, Shahididadras M, Diab R, Vahidnezhad F, Zeinali S, Beziat V, Jouanguy E, Casanova JL, Uitto J, Vahidnezhad H (2024). Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency. JID Innovations, 4(4), -.
Chicago	Youssefian L, Saeidian AH, Saffarian Z, Ariamanesh M, Abdollahimajd F, Molkara S, Shahididadras M, et al.. "Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency." JID Innovations 4, no. 4 (2024): -.
Harvard	Youssefian L et al. (2024) 'Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency', JID Innovations, 4(4), pp. -.
Vancouver	Youssefian L, Saeidian AH, Saffarian Z, Ariamanesh M, Abdollahimajd F, Molkara S, et al.. Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency. JID Innovations. 2024;4(4):-.
BibTex	@article{ author = {Youssefian L and Saeidian AH and Saffarian Z and Ariamanesh M and Abdollahimajd F and Molkara S and Shahididadras M and Diab R and Vahidnezhad F and Zeinali S and Beziat V and Jouanguy E and Casanova JL and Uitto J and Vahidnezhad H}, title = {Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency}, journal = {JID Innovations}, volume = {4}, number = {4}, pages = {-}, year = {2024} }
RIS	TY - JOUR AU - Youssefian L AU - Saeidian AH AU - Saffarian Z AU - Ariamanesh M AU - Abdollahimajd F AU - Molkara S AU - Shahididadras M AU - Diab R AU - Vahidnezhad F AU - Zeinali S AU - Beziat V AU - Jouanguy E AU - Casanova JL AU - Uitto J AU - Vahidnezhad H TI - Whole-Transcriptome Sequencing–Based Profiling of the Cutaneous Virome in Patients With Secondary Immunodeficiency JO - JID Innovations VL - 4 IS - 4 SP - EP - PY - 2024 ER -