Isfahan University of Medical Sciences

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Molecualr Cloning of the Capsular Antigen F1 of Yersinia Pestis in Pbad/Giii Plasmid



Jahaniannajafabadi A1 ; Soleimani M2 ; Azadmanesh K3 ; Mostafavi E4 ; Majidzadeha K2
Authors

Source: Research in Pharmaceutical Sciences Published:2015

Abstract

Yersinia pestis which is the causative agent of pneumonic plague and distributed in all continents has led to many deaths during the history. Because of its high mortality rate, it must be diagnosed and treated at the earliest time post infection and therefore, rapid diagnostic tests are required. In the present study, we cloned the coding sequence of F1 capsular antigen of the bacteria in the pBAD/gIII plasmid for later expression and purification of the protein to produce poly and monoclonal antibodies against this antigen, and subsequently to develop rapid and efficient diagnostics tools for Y. pestis infections.